FastQCFastQC Report
Fri 14 Jul 2023
SRR9691682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5445666
Total Bases413.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG1324862.432870469837849No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA1011871.858119833276591Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA921031.691308280750233No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA772451.418467456505779No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA721081.3241355602785774No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC375280.6891351764871367No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG315730.5797821607127577No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA286370.5258677267390252No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG281750.517383915943431No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG277230.5090837374161397No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA264940.4865153316417129Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA256380.47079640947498436No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA204610.37572998417457115No Hit
AAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCG202800.3724062401182886No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA187440.3442003237069626No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA159760.29337091184071884No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG157620.2894411812990367No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAA155050.2847218320036521No Hit
GGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAG154530.283766944208477No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA143780.26402647536591484No Hit
GGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCAC143700.2638795695512725No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG141220.2593254892973605Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA134420.24683849505276306No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG127330.23381896723008716No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG122120.2242517260515059No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGAGATCGGAAGA119100.2187060315487582No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA119000.2185223992804553No Hit
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA115820.21268289314842298No Hit
TTTCCGGCAGGCGAAGGTGAAACCTTTCAGCCGCTGACCTGGGAACTGAT114800.21080984401173336No Hit
AATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAG112140.20592522567487612No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC108410.19907574206717785No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC97210.17850892801725263No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG96960.17804984734649537No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAG96560.17731531827328376No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC79210.14545511972272998Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA77690.14266390924452582No Hit
TTAGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACT77500.14231500793475033Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA74770.13730184701008105Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCA73570.1350982597904462No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC70910.13021364145358896No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGAAGATCGGAAG70330.12914857429743212No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA69730.12804678068761471No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC67090.12319888880441805No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG66430.12198691583361887No Hit
AGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC66250.12165637775067364Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGA65160.119654786026172No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA64640.11869989823099691No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGA63990.11750628848702803No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG60230.11060171519883887No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA60200.11054662551834799No Hit
TAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCG60060.11028954034272391No Hit
AAAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGA59450.1091693835060762No Hit
AGCTCTTGATCCGGCAAACAAACCACCGCTAGATCGGAAGAGCACACGTC56760.10422967548872811No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA56330.10344005673502561Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA54840.10070393593731235No Hit
TATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTAT54500.10007958622508248No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)