FastQCFastQC Report
Fri 14 Jul 2023
SRR9691685_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691685_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5587875
Total Bases424.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1433772.56585911460081No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG804631.4399570498624255No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA592121.0596514775294723Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA479370.8578753103818535Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA457030.8178958906561081No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC443360.7934322081292083No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT395240.7073171822920162Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG264870.474008455808335No Hit
TTTTCGTGGTCTGAAACTGTGGATGGTTATGCGTACCAGATCGGAAGAGC253000.4527660335995347No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA244490.43753663065118675No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC208170.37253875578820217Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG203730.36459298033689014No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG196490.3516363555018679No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA191520.3427420978457822Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG189010.33825022929109905No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC181160.32420195512605415No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA163450.2925083327740868No Hit
TCGTTTTCGTGGTCTGAAACTGTGGATGGTTATGCGTACCAGATCGGAAG155150.277654743529517No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA149020.2666845625573228No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA145680.26070733507818267Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA145140.25974095698275285No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT141440.25311947743999286No Hit
ATCTGGATGGTGTGGAACGTGTTGATAGCATTAGCATGAGATCGGAAGAG134510.24071762521530993No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG133870.23957228821331902No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG131430.23520569089322865No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC128190.22940742232064965No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT121140.21679081940809342No Hit
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGA112520.20136456166252825No Hit
TGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTAGATCGGAAGAG110690.19808961367246045No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT110090.197015860233094Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA106240.19012594233049238No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG105070.18803212312372772No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA100770.18033689014160123No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC100360.1796031586247008No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG96120.17201530098651097No Hit
TTCGTGGTCTGAAACTGTGGATGGTTATGCGTACCAGATCGGAAGAGCAC92080.1647853611614433No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG91280.16335368990895466No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA90560.1620651857817149No Hit
TTTTCGTGGTCTGAAACTGTGGATGGTTATGCGAGATCGGAAGAGCACAC90070.16118828713956557No Hit
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA89670.16047245151332126No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA89610.1603650761693846No Hit
ATTGGCTGGCACAGATGCTGAATCTGCCGACCAGCTTTATGAATCGTACC87460.15651745967832137No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC86920.15555108158289152No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA82570.1477663691474845No Hit
ATGGTCTGGATCCGGCACGTCTGCTGGAGATCGGAAGAGCACACGTCTGA81990.14672840748943025Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA81750.14629890611368365No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG80240.1435966266246113No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC76490.1368856676285708No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA75540.13518555801624052No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA74390.13312753059078808No Hit
AAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACACGT74300.13296646757488312No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC74210.13280540455897816No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA71470.1279019305192045No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC70720.12655973871999643No Hit
TGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAGATCGGAAG69670.12468067020110507No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA69450.1242869606066707No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA69310.12403641813748519Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTGTCTGGATTGTACCTGTCTGTATGTTCGTGAGATCGGAAGAGCACA67560.12090463727266626No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA66090.11827394134621837No Hit
ACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGC64690.11576851665436325No Hit
AAACTGTGGATGGTTATGCGTACCAGATCGGAAGAGCACACGTCTGAACT64300.11507057691877504Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG62190.11129454399033621Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCGTGGTCTGAAACTGTGGATGGTTATGCGTACCAGATCGGAAGAGCA60710.10864595217323222No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA58390.10449410554101515No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAGATCGGAA58300.10433304252511018No Hit
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGG57460.10282978770999708No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC57170.10231080688096995Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCAC56780.10161286714538174No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA56330.10080755206585687No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)