FastQCFastQC Report
Fri 14 Jul 2023
SRR9691687_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691687_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5920379
Total Bases449.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1766592.9839136987682715No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA974861.6466175560720016Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG872501.4737232194087575No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG756921.2784992312147585No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA683251.1540646299839925No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA619151.0457945344377446No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA380770.6431513928415731No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA348620.5888474369630728Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAAGTATCTCGTATG336200.5678690502753286TruSeq Adapter, Index 3 (97% over 36bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG323270.5460292322501651No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA319650.5399147588355407No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG283710.47920918576327626No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA278930.4711353783262862Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG233610.39458622496971896No Hit
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA219340.37048303833251217No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC198200.3347758648559493No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC190510.321786831552507No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC183510.3099632641761617No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG178770.3019570199813222No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG152350.2573314985408873Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGTCTGGATCCGGCACGTCTGCTGGAGATCGGAAGAGCACACGTCTGA152350.2573314985408873Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA150880.2548485493918548No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG143470.24233245878346638No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA133920.22620173472002383No Hit
ATTGGCTGGCACAGATGCTGAATCTGCCGACCAGCTTTATGAATCGTACC132530.2238539120552924No Hit
TAAAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACAC122590.20706444638088203No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT121240.20478418695830114Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA120160.20295997942023644No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA110080.18593404239829917No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG101910.1721342501890504No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA100280.1693810480714157No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA97710.1650401097632432No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCACA93980.1587398374327049No Hit
ATCTGATTGCAAGCGCAATGAATACCGTTGGTTTTACCAGATCGGAAGAG93420.15779395204259727No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA92440.15613865260990895No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG91240.15411175534539256No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC91220.1540779737243173No Hit
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGG90240.15242267429162898No Hit
AGTTACCGATCTGCTTGATATGCAGGTTGGTGTTGGTCGTCGTTTTCGTG87080.14708517816173594No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA86790.1465953446561445Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTCGTGGTCTGAAACTGTGGATGGTTATGCGTACCAGATCGGAAGAGC85320.14411239550711194No Hit
TCGTTTTCGTGGTCTGAAACTGTGGATGGTTATGCGTACCAGATCGGAAG79660.13455219674280988No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT79530.1343326162058206Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA78540.13266042596259464No Hit
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGA78330.13230571894130427No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA78320.13228882813076664Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG77920.1316131957092612No Hit
GGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCAC76760.12965386168689538No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGATCGGAAGAGCA76720.12958629844474484No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG74880.12647838930581978No Hit
AAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACACGT74310.1255156131051745No Hit
AGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC71530.12081996777571166Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC70780.11955315698538894No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG67670.11430011490818409No Hit
ACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGC67510.1140298619395819No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA66360.11208741872775374No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGTAGATCGG65880.1112766598219472No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA63540.10732421015614034No Hit
TGGTCTGGATCCGGCACGTCTGCTGGAGATCGGAAGAGCACACGTCTGAA63000.10641210638710798Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATTTGTCCGGAATTTCGTCATCATCTGGATGGTGTGGAACGAGATCGGA62710.10592227288151654No Hit
TTAGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACT61290.10352377778517219Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC60820.10272990968990331No Hit
TCATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAA60690.10251032915291403No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)