FastQCFastQC Report
Fri 14 Jul 2023
SRR9691689_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691689_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5869005
Total Bases446 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1745572.974217946653649No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA971031.6545053207485765Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG862691.46990844274285No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG748121.2746964775119463No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA682801.1633999289487742No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA610131.0395799628727527No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA362750.6180775105831396No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAAGTATCTCGTATG358430.6107168080449752TruSeq Adapter, Index 3 (97% over 36bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA352650.6008684606675238Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA317470.540926443238675No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG317260.5405686313097364No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG277520.47285698342393645No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA275660.4696877920533378Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG230740.39315011658705346No Hit
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA215960.3679669722550927No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC197200.3360024399365821No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC184190.3138351390056747No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG179620.30604846988544054No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC177850.30303262648438706No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG153160.2609641668391831Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA150670.25672153968176886No Hit
ATGGTCTGGATCCGGCACGTCTGCTGGAGATCGGAAGAGCACACGTCTGA148630.2532456523720801Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG141600.24126747208428004No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA134130.22853959061203732No Hit
ATTGGCTGGCACAGATGCTGAATCTGCCGACCAGCTTTATGAATCGTACC128690.21927055778620058No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT122490.20870658655087193Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACAC119550.2036972195457322No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA117920.20091991743063772No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA110120.187629760070063No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG100500.1712385659920208No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA98100.16714928680415164No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCACA93960.16009528020507735No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA92110.15694312749776154No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA91990.15673866353836807No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC91440.15580153705781474No Hit
ATCTGATTGCAAGCGCAATGAATACCGTTGGTTTTACCAGATCGGAAGAG91360.15566522775155245No Hit
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGG88820.15133740727772424No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG87560.14919053570409294No Hit
AGTTACCGATCTGCTTGATATGCAGGTTGGTGTTGGTCGTCGTTTTCGTG86040.14660065888510915No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA85780.14615765363975666Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTCGTGGTCTGAAACTGTGGATGGTTATGCGTACCAGATCGGAAGAGC83350.14201725846203914No Hit
TCGTTTTCGTGGTCTGAAACTGTGGATGGTTATGCGTACCAGATCGGAAG80790.13765536066164535No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG78980.13457136260746072No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT78950.13452024661761236Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA76970.1311465912876203No Hit
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGA76950.13111251396105472No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGATCGGAAGAGCA76780.13082285668524732No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA76780.13082285668524732Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACACGT76500.13034577411332926No Hit
GGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCAC73490.12521713646521002No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG71490.1218094038086524No Hit
AGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC70520.12015665347022196Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC68820.11726008071214797No Hit
ACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGC67100.1143294306275084No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG66470.11325599484069276No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA65930.11233590702342221No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA65160.1110239299506475No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGTAGATCGG65110.11093873663423356No Hit
TGGTCTGGATCCGGCACGTCTGCTGGAGATCGGAAGAGCACACGTCTGAA62920.10720726937530298Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATTTGTCCGGAATTTCGTCATCATCTGGATGGTGTGGAACGAGATCGGA60860.10369730473904862No Hit
TCATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAA59680.10168674247167961No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC59400.10120965989976155No Hit
TTAGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACT58850.10027253341920819Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)