FastQCFastQC Report
Fri 14 Jul 2023
SRR9691690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5724108
Total Bases435 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1730363.0229338789554636No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA967891.6909010102534754Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG848661.4826065476053212No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG733111.2807410342362513No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA657741.1490698638110952No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA605671.0581037255062273No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA364480.6367454981632072No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA350490.612305008920167Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAAGTATCTCGTATG348040.6080248660577333TruSeq Adapter, Index 3 (97% over 36bp)
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA318480.5563836321746549No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG315220.550688421671988No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG278480.48650374870634866No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA273980.4786422618161642Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG233320.40760936027063077No Hit
ATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACA215140.3758489532342856No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC191280.3341656027454409No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC186490.32579748670011116No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC176690.30867691525037616No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG176400.3081702860952309No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG150980.26376162015112226Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA145680.25450253559157165No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG135870.23736449417096953No Hit
ATGGTCTGGATCCGGCACGTCTGCTGGAGATCGGAAGAGCACACGTCTGA130510.22800058978621646Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGGCTGGCACAGATGCTGAATCTGCCGACCAGCTTTATGAATCGTACC127740.22316140785603625No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA127590.22289935829303012No Hit
TAAAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACAC118610.20721132445439533No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT118340.20673963524098427Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA116820.20408419966918864No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA106980.18689374833598527No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG97800.17085631508000898No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA96420.1684454591003524No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA95390.16664605210104352No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCACA93800.16386832673317833No Hit
ATCTGATTGCAAGCGCAATGAATACCGTTGGTTTTACCAGATCGGAAGAG89100.15565744042565235No Hit
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGG88980.15544780077524742No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC88680.15492370164923513No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA87910.15357851389247024No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG87480.15282730514518594No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA84650.14788330338980327Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTACCGATCTGCTTGATATGCAGGTTGGTGTTGGTCGTCGTTTTCGTG84060.14685257510864574No Hit
TTTTCGTGGTCTGAAACTGTGGATGGTTATGCGTACCAGATCGGAAGAGC83920.14660799551650666No Hit
TCGTTTTCGTGGTCTGAAACTGTGGATGGTTATGCGTACCAGATCGGAAG79950.1396724170822773No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT77340.13511275468597028Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG76610.1338374468126737No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA76180.13308623806538938No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA75150.1312868310660805Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGA74690.13048321240619498No Hit
AAGACTGACTAAGCATGTAGTACCGAGGATGAGATCGGAAGAGCACACGT74370.12992417333844852No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGATCGGAAGAGCA73540.12847416575648118No Hit
GGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCAC72860.12728620773751997No Hit
AGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC71230.12443860248618649Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG70690.12349522405936436No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC66070.11542409751877497No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG65220.11393914999507347No Hit
ACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGC64390.1124891424131061No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA63000.11006081646258245No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGTAGATCGG62950.10997346660824708No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA61690.10777225027899544No Hit
TGGTCTGGATCCGGCACGTCTGCTGGAGATCGGAAGAGCACACGTCTGAA61000.10656682228916715Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATTTGTCCGGAATTTCGTCATCATCTGGATGGTGTGGAACGAGATCGGA60680.10600778322142071No Hit
TTAGGACGGGGATCAAGAGAGGTCAGATCGGAAGAGCACACGTCTGAACT60530.10574573365841455Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC59840.10454030566858627No Hit
TCATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAA59010.1030902980866189No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)