FastQCFastQC Report
Tue 18 Jul 2023
SRR9691693_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691693_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5199895
Total Bases395.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA563821.084291125109257No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG487150.9368458401563877No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA409160.7868620424066256No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA336510.6471476827897487Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGG316800.6092430712543234No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA247870.47668270224687226No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG243480.46824022408144783No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG200180.384969311880336No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA169880.32669890449711003No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA165220.3177371850777756No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG164800.3169294764605824No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC156710.3013714700008366No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG149620.28773657929631274No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA147780.28419804630670426No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA120590.23190852892221864No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC118580.22804306625422247No Hit
AATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGGAAGAGCACAC117760.22646611133494043No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA115860.22281219140001868Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG111250.2139466277684453No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG106950.20567723002099084No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC105400.20269640060039673No Hit
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCTGAGATCGGAA103320.198696319829535No Hit
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGA95360.18338831841796804No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG92230.17736896610412325No Hit
ATTGGCTGGCACAGATGCTGAATCTGCCGACCAGCTTTATGAATCGTACC81550.1568300898383525No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA76870.14782990810391364No Hit
GGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCAC76080.14631064665728827No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC74780.14381059617549968No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC74620.14350289765466417No Hit
AAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCG74330.14294519408564982No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA73680.1416951688447555No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA69040.13277191174052552No Hit
ACCTGTCTGGATTGTACCTGTCTGTATGTTCGTGAGATCGGAAGAGCACA68650.13202189659598895No Hit
AATGGCTGATGACCTGTCTGGATTGTACCTGTCTGAGATCGGAAGAGCAC67880.13054109746446804No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG67680.13015647431342364No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA67150.129137222963156No Hit
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCTGTCTAGATCG66580.12804104698267946No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA63870.1228294032860279No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA63140.12142552878471585No Hit
ATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGGAAGAGCACACG61020.11734852338364525No Hit
AAATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGGAAGAGCACA58860.11319459335236577No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT58250.11202149274168036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG54880.10554059264658228No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA53060.10204052197207829No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA52650.10125204451243727No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)