FastQCFastQC Report
Fri 14 Jul 2023
SRR9691694_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691694_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4966828
Total Bases377.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA538791.0847768434904532No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG470040.9463585209715335No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA385010.7751627396801339No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA330110.664629417406844Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGG305600.6152820270804626No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA238970.48113202228867197No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG235290.47372286698875016No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG185140.3727529924531311No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA156290.3146676309306463No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA154590.31124492331926934No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC153550.3091510316040741No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG153480.30901009658478207No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG144720.2913730855990987No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA141040.2839639302991769No Hit
AATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGGAAGAGCACAC115680.23290518616710704No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA113760.2290395399236696Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA113380.22827446410465593No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC112990.2274892547114577No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG105780.21297294772438263No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG102400.20616779964999793No Hit
ATGGCGTCGGCAAGACCCGTTCTAAAGCCATCCTGGCTGCAGCGGGTATC100390.20212095123889937No Hit
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCTGAGATCGGAA99530.2003894638590263No Hit
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGA91290.18379939873094053No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG87550.17626944198591135No Hit
ATTGGCTGGCACAGATGCTGAATCTGCCGACCAGCTTTATGAATCGTACC80630.16233700865018882No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA76180.15337756813805511No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA74180.1493508533011411No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC71790.14453892907102883No Hit
AAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCG71450.14385438754875346No Hit
GGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCAC71270.1434919832134312No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC70550.14204236587214214No Hit
AATGGCTGATGACCTGTCTGGATTGTACCTGTCTGAGATCGGAAGAGCAC67340.13557948855889512No Hit
ACCTGTCTGGATTGTACCTGTCTGTATGTTCGTGAGATCGGAAGAGCACA66430.13374733330809926No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG65730.13233798311517933No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA64620.13010315638069206No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA64110.12907634409727897No Hit
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCTGTCTAGATCG63600.1280495318138659No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA61380.12357987834489136No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA60730.12227119602289428No Hit
ATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGGAAGAGCACACG59690.120177304307699No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT57260.11528484578084847Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGGAAGAGCACA56050.11284868330451547No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG50980.10264096119293843No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA50470.10161414890952536No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC49700.10006386369731345No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)