FastQCFastQC Report
Fri 14 Jul 2023
SRR9691695_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691695_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6096422
Total Bases463.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATCTCGTATG1736802.8488841487679166TruSeq Adapter, Index 10 (97% over 36bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG833171.3666540800489206No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA819241.3438046119510756No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA768411.260427837836685Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA589430.9668457990604982No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG386680.6342736772487206No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA310070.5086098042425541No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA281060.461024515691335No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA276190.4530362235422679Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG271730.44572045701560686No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG234190.3841433549055495No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA199000.3264209728263562No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC192980.3165463283217599No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATCTCGTAT178250.29238461510702507TruSeq Adapter, Index 10 (97% over 36bp)
AAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCG141230.23166047232294615No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG130340.21379753566928275No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA126090.20682623348580528No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG119670.19629546642276405No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA113040.1854202350165392No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA107820.17685783562883278No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC106890.17533235068044828No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA105920.17374125347621933No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG99410.16306285883752797Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA98460.16150456776122124Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC95830.1571905619394458No Hit
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGG94580.15514017894430537No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA94510.1550253574965775No Hit
GGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCAC93010.15256489790240899No Hit
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA89180.1462825244052987No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG84830.13914719158221003No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC77450.12704173037890093No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGA74710.1225472908535531No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG74660.1224652755337475No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA74570.1223176479580974No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATATCGTATG73910.12123504573666324TruSeq Adapter, Index 10 (97% over 36bp)
AGCTCTTGATCCGGCAAACAAACCACCGCTAGATCGGAAGAGCACACGTC72790.11939790257301743No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA71590.11742953489768261No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAA67520.11075348786550537No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG67040.10996614079537145No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA66850.1096544825801101No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATAGATCGG66390.10889994163789843No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATGTCGTATG65300.10711200766613599TruSeq Adapter, Index 10 (97% over 36bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC65180.1069151708986025No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG63130.10355254278657218No Hit
ATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGA61790.10135453221578165No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC61560.10097726174467582No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)