FastQCFastQC Report
Fri 14 Jul 2023
SRR9691697_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691697_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6749047
Total Bases512.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATCTCGTATG1940162.874716978560084TruSeq Adapter, Index 10 (97% over 36bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG946801.4028647303834156No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA906421.3430340609570508No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA851801.2621041163293127Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA653680.9685515599461672No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG427560.6335116646839176No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA341980.5067085767812848No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA324330.4805567363807068No Hit
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA304140.4506414016675243Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG301410.4465963861268117No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG263360.39021805597145787No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA221480.3281648505337124No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC211910.3139850707811044No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATCTCGTAT195310.2893890055884927TruSeq Adapter, Index 10 (97% over 36bp)
AAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCG159030.23563326792656802No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA143990.21334864018579214No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG141090.2090517372304564No Hit
TCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAG133720.19813167696120654No Hit
AGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCA125450.18587809508512831No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC119810.17752135968233737No Hit
TGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAGATCGGA117940.17475059812148294No Hit
ACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCA115930.17177239986623297No Hit
TGTGGGCACTCGAAGATACGGATTCTTAAGATCGGAAGAGCACACGTCTG109360.16203769213638605Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGG107360.15907431078787865No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA106830.15828901473052417No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA106600.15794822587544582Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGCTAAAGATGAGATCGGA103820.1538291258010205No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC103310.1530734635571511No Hit
GGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCAC97030.1437684461228378No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG94300.13972343058212516No Hit
AGCTCTTGATCCGGCAAACAAACCACCGCTAGATCGGAAGAGCACACGTC86030.12746984870604694No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGA83780.12413604468897609No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC82300.1219431424910806No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG82050.12157271982251718No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA81950.12142455075509179No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCAGATCGGAAGA77990.1155570556850471No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGA75360.11166020921175983No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATAGATCGG74910.11099344840834566No Hit
AAATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAA73380.10872646167673747No Hit
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG72330.10717068646877106No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATGTCGTATG71540.10600015083611064TruSeq Adapter, Index 10 (97% over 36bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTAGATCGGAAGAGCAC70780.10487406592367783No Hit
ATCGATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGA70320.1041924882135211No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTCAATATCGTATG70040.10377761482473008TruSeq Adapter, Index 10 (97% over 36bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAAGATCGGAAGAGCACAC69770.10337755834268157No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG69350.102755248259495No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)