FastQCFastQC Report
Fri 14 Jul 2023
SRR9691701_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691701_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6465123
Total Bases491.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG1250231.9338069824812305No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC1150971.780275487411454No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA1115501.72541187538118No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1072951.6595971955985989Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA802561.241368493685271No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC748421.1576268541217236No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG672821.0406917238852222No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA498340.7708128677520907No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG468390.7244873763422598No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC460790.7127319928793311No Hit
ATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGAGCACACGTCTG432040.6682626146478574Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT328010.5073530696941111Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC306990.47484015385322137No Hit
AATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGAGCACACGTCT277470.42917976966563515Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG261670.40474094615059913Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA250650.38769564012935254No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC246860.3818334160077078Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCTGTCTGGATTGTACCTGTCTGTATGTTCGTGAGATCGGAAGAGCACA222580.34427805936561456No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG194690.30113889557863016No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC183930.28449574741269423Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACATATCTCGTATG171870.26584180997020473TruSeq Adapter, Index 4 (97% over 37bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA163190.25241592464675455No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA161680.25008031556398846No Hit
AATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGGAAGAGCACAC154590.23911378020186158No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC139260.2154019343483488Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC132820.2054407936244987No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT125980.19486094850786287Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC117310.18145053079423237Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG116460.1801357839595627No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC115420.17852715253831986No Hit
ATGCCGGTCTGGTGCCGACCTATGTTTGTGCAGATCGGAAGAGCACACGT112680.17428902744773767No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT108690.16811745112970009No Hit
TTCGTGATACCCATCGTCTGACCGGTTCAGATCGGAAGAGCACACGTCTG108070.16715845932088222Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGGAAGAGCACACG107320.165998388584409No Hit
ACCTGTCTGGATTGTACCTGTCTGTATGTTCGAGATCGGAAGAGCACACG103150.15954839528961784No Hit
ATTGGCTGGCACAGATGCTGAATCTGCCGACCAGCTTTATGAATCGTACC101370.1567951607417214No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA101110.15639300288641067Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGCTGATGACCTGTCTGGATTGTACCTGAGATCGGAAGAGCACACGTCT96810.14974193066396416Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG94400.1460142366974302No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC90600.14013654496596584No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT83720.12949482941005144Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATGGCTGATGACCTGTCTGGATTGTACCTGAGATCGGAAGAGCACACGT79600.12312217416435851No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG75360.11656390760082987No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA75340.11653297238119059No Hit
AACTGATGGAACGTCTGAATAAAACCGGTAAAGCAGATCGGAAGAGCACA74040.11452218310463698No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG73400.11353225607617984TruSeq Adapter, Index 4 (100% over 35bp)
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG71560.11068621586936551No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA71250.11020671996495657Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGACCTGTCTGGATTGTACCTGTCTGTATGTTCGTGAGATCGGAAGAGC69380.10731427692868335No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT68600.10610780336275118Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGCTGATGACCTGTCTGGATTGTACCTGAGATCGGAAGAGCACACGTC66970.10358658296214937No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)