FastQCFastQC Report
Fri 14 Jul 2023
SRR9691702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6153385
Total Bases467.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG1188341.9311972190916058No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC1098151.7846274855221962No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA1066651.7334361493714436No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1034851.681757276685922Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA753821.2250493021320785No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC710191.1541452387588296No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG642271.043766967287111No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA472200.7673825057265229No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG448250.7284608390341252No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC434110.7054816170286761No Hit
ATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGAGCACACGTCTG403770.656175422145697Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT310180.5040802745155716Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC293790.47744452849935437No Hit
AATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGAGCACACGTCT263870.4288208847650521Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG250500.407093006532177Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA240090.39017548877569014No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC231610.37639445605955096Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCTGTCTGGATTGTACCTGTCTGTATGTTCGTGAGATCGGAAGAGCACA210880.342705681507008No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG189530.30800933144927545No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC172730.28070728550220736Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACATATCTCGTATG163290.2653661358748071TruSeq Adapter, Index 4 (97% over 37bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA154460.2510163105347707No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA153430.24934243509873022No Hit
AATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGGAAGAGCACAC146290.23773906557122623No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC133410.21680749701180735Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC127790.20767431259379998No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT120200.1953396382641424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC112860.18341124438012574Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG110340.1793159374880655No Hit
ATGCCGGTCTGGTGCCGACCTATGTTTGTGCAGATCGGAAGAGCACACGT106820.1735955088134417No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC105710.17179162363479614No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT105390.17127158466437578No Hit
TTCGTGATACCCATCGTCTGACCGGTTCAGATCGGAAGAGCACACGTCTG102190.16607119496017234Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGGAAGAGCACACG101520.16498236336585473No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA96040.1560766959974063Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTGTCTGGATTGTACCTGTCTGTATGTTCGAGATCGGAAGAGCACACG95660.15545914972003214No Hit
ATTGGCTGGCACAGATGCTGAATCTGCCGACCAGCTTTATGAATCGTACC95170.154662840046576No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG91880.1493161893819418No Hit
TGGCTGATGACCTGTCTGGATTGTACCTGAGATCGGAAGAGCACACGTCT90350.1468297530546195Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC86820.1410930731621701No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT78760.1279945915947076Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATGGCTGATGACCTGTCTGGATTGTACCTGAGATCGGAAGAGCACACGT74580.12120158254359185No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA73050.1187151462162696No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG71470.11614745379981911No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG69740.11333599311598413TruSeq Adapter, Index 4 (100% over 35bp)
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG68410.11117458114517456No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA68360.11109332505604638Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTGATGGAACGTCTGAATAAAACCGGTAAAGCAGATCGGAAGAGCACA68150.11075204948170804No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT67690.1100044934617288Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGACCTGTCTGGATTGTACCTGTCTGTATGTTCGTGAGATCGGAAGAGC65530.10649423041139146No Hit
ATGGCTGATGACCTGTCTGGATTGTACCTGAGATCGGAAGAGCACACGTC63320.10290271127192593No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)