FastQCFastQC Report
Fri 14 Jul 2023
SRR9691705_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691705_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5254421
Total Bases399.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1744923.320860661907373Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1262582.4028908227947476No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG1095632.08515838376864No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC817521.5558707610220042No Hit
ATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGAGCACACGTCTG513960.9781477350216132Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGAGCACACGTCT384540.7318408631512398Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG366570.6976410911877826No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG355110.6758308860291172Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT256410.48798906673066356No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC250520.4767794586691855Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA241520.4596510252985058No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC223150.4246899896296852No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACGGATCTCGTATG209980.3996253821305906TruSeq Adapter, Index 4 (97% over 37bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA191820.3650640099070858No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA186940.3557765927016507No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG162400.30907306437759746Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGGAAGAGCACAC161150.3066941152983364No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC139780.26602360183928925Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA139150.2648246115033417Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT133390.2538624141461067Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA127080.24185347919399686No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC125850.23951259330000393Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA122390.2329276622486093No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG122260.23268025154436614No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA121990.23216639854324578Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA116290.22131839074181534Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC115410.21964361059001553No Hit
ACCTGTCTGGATTGTACCTGTCTGTATGTTCGTGAGATCGGAAGAGCACA113170.21538053383997968No Hit
ATTGGCTGGCACAGATGCTGAATCTGCCGACCAGCTTTATGAATCGTACC112150.21343931139130268No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC100960.19214295923375763No Hit
TGGCTGATGACCTGTCTGGATTGTACCTGAGATCGGAAGAGCACACGTCT97690.1859196284424107Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA96790.18420678510534272No Hit
AAATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGAGCACACGTC94050.17899212872360248No Hit
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA92620.1762706109769278Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC88560.16854378436748788No Hit
ATGCCGGTCTGGTGCCGACCTATGTTTGTGCAGATCGGAAGAGCACACGT87790.16707835173466304No Hit
ATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGGAAGAGCACACG85590.16289140135516358No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT84780.1613498423518024Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG83810.15950377786629583TruSeq Adapter, Index 11 (100% over 35bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA80630.1534517314086557Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG79430.15116794029256506No Hit
TTCGTGATACCCATCGTCTGACCGGTTCAGATCGGAAGAGCACACGTCTG78740.14985476040081297Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC77300.1471142110615042No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC73280.1394635108226006No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA72640.13824548889401897Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG72640.13824548889401897No Hit
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG67190.12787327090844072Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATGGCTGATGACCTGTCTGGATTGTACCTGAGATCGGAAGAGCACACGT65850.12532303749547286No Hit
TCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAAC63930.12166897170972786Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC63060.12001322315056215No Hit
AGAATGTGGCGCTCCGCGGTTGAGATCGGAAGAGCACACGTCTGAACTCC62180.11833844299876238Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG60730.11557886206681955No Hit
ACGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG60680.1154837041036491TruSeq Adapter, Index 11 (100% over 35bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG60250.11466534562038329No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC58800.11190576468844046No Hit
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA58600.11152513283575868No Hit
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGA58580.11148706965049052No Hit
ACCTGTCTGGATTGTACCTGTCTGTATGTTCGAGATCGGAAGAGCACACG57900.11019292135137249No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA57600.10962197357234985Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGCTGATGACCTGTCTGGATTGTACCTGAGATCGGAAGAGCACACGTC57530.1094887524239112No Hit
ATGACCTGTCTGGATTGTACCTGTCTGTATGTTCGTGAGATCGGAAGAGC57120.10870845712591358No Hit
ATGACCTGTCTGGATTGTACCTGTCTGTATGAGATCGGAAGAGCACACGT56860.10821363571742729No Hit
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT56670.1078520354573796Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGGCACTCGAAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC55850.10629144486138434Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCCCGAGATCGGAAGAGC54280.10330348481783244No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA53450.1017238626292031No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA53080.10101969370174183Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)