FastQCFastQC Report
Fri 14 Jul 2023
SRR9691706_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691706_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5004151
Total Bases380.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA1670933.339087889234358Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1203382.404763565288098No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG1048762.095780083374782No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC777331.553370391900644No Hit
ATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGAGCACACGTCTG480710.9606224912077993Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGAGCACACGTCT354990.7093910635390499Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG345390.6902069901567718No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG341340.6821137091986234Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT247420.4944295246086699No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC235720.4710489351740186Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA232540.46469421086613893No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC211650.42294886784991104No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACGGATCTCGTATG194820.38931678920160484TruSeq Adapter, Index 4 (97% over 37bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA181650.36299863853029213No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA176750.35320676774142107No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG153920.3075846432291911Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGGAAGAGCACAC151860.30346806081591066No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA134310.2683971766639336Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC133580.26693838775048956Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT127990.2557676616872672Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA121530.242858378973776No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC119830.2394611993123309Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG117490.23478508142540067No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA115830.23146783540304838Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA115500.2308083828805326No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC111550.2229149360201161No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA108840.2174994319715772Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGGCTGGCACAGATGCTGAATCTGCCGACCAGCTTTATGAATCGTACC104820.20946610124274828No Hit
ACCTGTCTGGATTGTACCTGTCTGTATGTTCGTGAGATCGGAAGAGCACA104150.20812721278794344No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC96580.1929997715896263No Hit
TGGCTGATGACCTGTCTGGATTGTACCTGAGATCGGAAGAGCACACGTCT92310.1844668556164672Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA90740.18132946028207383No Hit
AAATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGAGCACACGTC90410.18067000775955802No Hit
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA89540.17893145110928907Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC83370.16660168727922078No Hit
ATGCCGGTCTGGTGCCGACCTATGTTTGTGCAGATCGGAAGAGCACACGT83240.1663419029521691No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT82470.16480318039963224Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGCTGATGACCTGTCTGGATTGTACCTGTCAGATCGGAAGAGCACACG82370.16460334630190018No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG78990.15784895379855643TruSeq Adapter, Index 11 (100% over 35bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA75370.15061495946065576Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG74550.14897631985925286No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC74390.14865658530288156No Hit
TTCGTGATACCCATCGTCTGACCGGTTCAGATCGGAAGAGCACACGTCTG74370.14861661848333516Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG72330.14454000288960106No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC70630.141142823228156No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA67750.13538760121347257Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGGCACTCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG66330.13254995702567726Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC62560.1250162115411785No Hit
TCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAAC61130.12215858394361001Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGCTGATGACCTGTCTGGATTGTACCTGAGATCGGAAGAGCACACGT60930.12175891574814589No Hit
AGAATGTGGCGCTCCGCGGTTGAGATCGGAAGAGCACACGTCTGAACTCC59820.11954075726331999Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG59800.11950079044377357No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG59200.1183017858573812No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC57040.11398536934636863No Hit
AGTCCGCATAAATGGCTGATGACCTGTCTGGATTGTACCAGATCGGAAGA56790.11348578410203848No Hit
ATGACCTGTCTGGATTGTACCTGTCTGTATGTTCGTGAGATCGGAAGAGC56720.11334590023362603No Hit
ACGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG56660.1132259997749868TruSeq Adapter, Index 11 (100% over 35bp)
AAACTGCGACGACACATTCACCTTGAACCAAGGGTTCAAGGGAGATCGGA56440.11278636475997626No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA54690.10928926804966517Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTGTCTGGATTGTACCTGTCTGTATGTTCGAGATCGGAAGAGCACACG54570.1090494671323867No Hit
ATGGCTGATGACCTGTCTGGATTGTACCTGAGATCGGAAGAGCACACGTC53510.10693122569642682No Hit
ATTTGCTCTTTAAAAATCTGGATCAGATCGGAAGAGCACACGTCTGAACT53380.10667144136937515Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGACCTGTCTGGATTGTACCTGTCTGTATGAGATCGGAAGAGCACACGT52610.10513271881683826No Hit
AATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCCCGAGATCGGAAGAGC52290.10449324970409565No Hit
TGTGGGCACTCGAAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC51770.10345411239588893Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA51060.1020352903019913No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)