FastQCFastQC Report
Fri 14 Jul 2023
SRR9691710_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691710_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4863743
Total Bases369.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC720101.4805469779139235No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA521081.0713559495228264Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA519021.0671205283667333No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC481390.9897521312289732No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA448310.9217386691689918No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG395900.8139821532511072No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG360220.7406230140038238No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG357500.7350306132540309No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA357170.734352123457181No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA211190.4342129096870455No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC183760.377816015361009Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTTTCGTCCGGTTACCGGTCTGCTGAGCGGTCGAGATCGGAAGAGCACA156400.3215630431130921No Hit
AGTTTAGCAAAAGCACCCTGGATGCCGGTGATCAGCTGGAAAGCGATCAT154770.31821171472259124No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC151080.3106249651759972No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA137140.2819639113333085No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT134360.27624814880227017No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC129540.2663380857088872No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT127000.26111577030283056Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA113890.23416122110070373No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA106480.2189260411168929No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG95250.19583682772712294No Hit
TTTAGCAAAAGCACCCTGGATGCCGGTGATCAGCTGGAAAGCGATCATCC90970.1870370206649488No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA90260.18557723958687783Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC87300.1794913917121032No Hit
ATCGGCATCTGTATGAACATACCGATAATCTGTGAGATCGGAAGAGCACA82950.17054766257180942No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA74840.1538732618068019No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA72510.14908271263510428Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT71530.1470678035414289No Hit
TCTGAACCCGGATTTTGCAGATTTTACCCAGTATATTGGTAGATCGGAAG67950.13970721726045146No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC66370.13645869035432176No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA64610.13284007810445575No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCATCTCGTATG64160.13191486474511502TruSeq Adapter, Index 7 (97% over 49bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG63190.12992051594831389No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA62840.12920090555771552No Hit
AGTTTCGTCCGGTTACCGGTCTGCTGAGCGGAGATCGGAAGAGCACACGT62200.12788504655776425No Hit
AGTTTCGTCCGGTTACCGGTCTGCTGAGCGGTCGTGAGATCGGAAGAGCA61330.1260963007297055No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG58310.11988709107368543Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT56540.1162479185269452Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTGTATGGACTGACGAAGAATCAGATCGGAAGAGCACACGTCTGAAC54810.11269098716770191Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT53010.10899013373033896Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGGATTTTGCAGATTTTACCCAGTATATTGGAGATCGGAAGAGCAC52170.1072630687929029No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)