FastQCFastQC Report
Fri 14 Jul 2023
SRR9691713_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691713_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5586902
Total Bases424.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA1592512.8504348205857197No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG758201.3571027377963674No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA484690.8675469875791628Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC450590.8065113724923044No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA398450.7131859481336884No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA396820.7102684099345219Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC320870.5743254490592461No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG316100.5657876225500286No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC311080.55680232085689Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA299730.5364869475068651No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA269250.4819307730831864Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT241520.4322968256826413Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA227790.4077214885816863No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA186510.3338343862126094No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC175550.31421707414950184Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA154250.2760921884794113Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA149920.26834191829389525No Hit
TCTGAACCCGGATTTTGCAGATTTTACCCAGTATATTGGTCAGATCGGAA136740.24475102659756695No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA122560.21937023416555365No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC121980.21833209173885634No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG118730.2125149143478801No Hit
TCTGAACCCGGATTTTGCAGATTTTACCCAGTATATTGGTCTGGCAGATC111700.19993191217601455No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC111320.19925174989645425No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA107010.1915372777256519No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA103190.1846998569153352Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA99750.17854259838457878No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG99600.17827411327422604No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA99180.17752235496523833No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT95770.17141879345655248No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT94090.16841176022060167Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTTCGTCCGGTTACCGGTCTGCTGAGCGGTCGAGATCGGAAGAGCACA89680.16051829797623082No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT89370.1599634287481685No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATCTCGTATG88910.1591400744097534TruSeq Adapter, Index 10 (97% over 38bp)
TCTGAACCCGGATTTTGCAGATTTTACCCAGTATATTGGTAGATCGGAAG86920.15557817194574022No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA86660.15511279775446213No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG86310.15448633249697238No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC86120.15414625135719223No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAA85730.15344819007027508No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG79860.14294147275180413No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG79750.14274458367087878No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA79340.14201072436924791Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC76930.1376970635962471Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG75810.13569237477227988No Hit
AGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGA73560.13166509811698865No Hit
AGTTTCGTCCGGTTACCGGTCTGCTGAGCGGTCGTGAGATCGGAAGAGCA70490.12617010285843566No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG70370.12595531477015348No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA70300.12583002171865554No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG69020.12353894877697871No Hit
TCCGTGTATGGACTGACGAAGAATCAGATCGGAAGAGCACACGTCTGAAC68950.12341365572548077Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGGGCACTCGAAGATACGGATTCTAGATCGGAAGAGCACACGTCTGAA68930.12337785771076708Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCAGATCGGAAGAGCACACGT67420.12067510759988273No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT66940.11981595524675392Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC66470.11897470190098197Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG65250.11679102300344626No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA64860.11609296171652914Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG62590.11202988704652418No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC62200.11133182575960703No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAA58890.10540725432448966TruSeq Adapter, Index 7 (97% over 35bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA57370.10268660520624848Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA56640.10137997766919843Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG56140.10048502730135593Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)