FastQCFastQC Report
Fri 14 Jul 2023
SRR9691715_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691715_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202645
Total Bases167.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG722163.2786036787589463No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG472352.1444672200922072No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT141300.6415014675537819No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA114490.5197841685791401No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC107950.49009259322314763No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA88390.4012902669290785No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC86230.39148387506838367No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA82430.3742318893875318No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA68620.31153454142633064No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG64250.291694757893351No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC64060.29083215860930833No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT62330.28297796512828893No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA59090.2682683773372468Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCT56850.2580987857780078Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT52610.23884920175516253No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGAGATCGGAAGAGCACACGTCTGAA51820.23526260473203808Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA49400.2242758138510745No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC46400.2106558251556651No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAAG45850.20815882722817342No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG43980.19966903427470153No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA42130.19127004124586577No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA41620.18895464316764618No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA39300.1784218519098629Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCAGACTCTGAACGCTAAAGTTGAGATCGGAAGAGCACACGTCTGAACT38890.17656045345482363Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT37870.17192965729838444TruSeq Adapter, Index 22 (100% over 36bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCAGATCGGAAGAGCACACGT37870.17192965729838444No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA37010.16802526053903377No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC36490.16566446249849612No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG35480.16107906630437496No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAAGATCGGAAGAGCACACGT34950.1586728683015193No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC33850.15367887244653586No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG31790.14432648020902142TruSeq Adapter, Index 2 (100% over 35bp)
TTCTTGGACTGGACCATAAAATTTCAGATCGGAAGAGCACACGTCTGAAC30980.14064908326126088Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30650.13915088450476587No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA30580.13883308476853964No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC29160.1323862901193792Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCTGCAGTTCAGTCGGTAGAACGAGATCGGAAGAGCACACGTCTGAA29130.1322500902324251Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC28780.13066109155129402No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTAAGATCGGAAGAGCACACG28660.13011629200347766No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG26810.12171729897464185No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA25220.11449870496607487No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAGATCGGAAGAGCAC25110.11399930538057654No Hit
ATATCTTCAGGAGATCGTCATGGACAGATTCCCACGAGATCGGAAGAGCA24900.11304590617189787No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG24540.11141150752844875Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG24530.11136610756613072No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA22450.10192291540398021Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)