FastQCFastQC Report
Fri 14 Jul 2023
SRR9691716_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691716_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265273
Total Bases172.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG758513.3484264369018657No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG510882.2552690117261807No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT149400.6595231568115631No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA120190.5305762263532916No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC114670.5062083024871616No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA94010.4150051671476242No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC92000.4061320644355007No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA88310.3898426370684681No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA73370.3238903213873118No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC68500.3023918088459978No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG67540.2981539090431926No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT65450.2889276480141687No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA61220.2702544020080582Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCT58450.2580262952853806Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT55820.2464162156172788No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGAGATCGGAAGAGCACACGTCTGAA54170.2391323253312073Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA51050.22535915097209036No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAAG48120.2124247276156119No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC47670.21043821208304694No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG46990.20743636638939325No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA44320.19564970756284122No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA43600.1924712827107373No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA41100.1814350853075987Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCAGACTCTGAACGCTAAAGTTGAGATCGGAAGAGCACACGTCTGAACT41040.18117021656992335Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT40260.17772692298014411TruSeq Adapter, Index 22 (100% over 36bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCAGATCGGAAGAGCACACGT39430.1740629054423021No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA38630.17053132227329773No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG37840.16704388389390595No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC37690.16638171204971763No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAAGATCGGAAGAGCACACGT35510.15675814791418075No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC35290.15578696254270455No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG32050.1414840507082369TruSeq Adapter, Index 2 (100% over 35bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA31940.14099845802249883No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGAGATCGGAAGAGCACACGTCTGAA31740.14011556223024774Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTTGGACTGGACCATAAAATTTCAGATCGGAAGAGCACACGTCTGAAC31490.13901194248993387Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC30140.13305239589223905No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTAAGATCGGAAGAGCACACG29290.1293000887751719No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC29280.12925594398555937Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG27880.12307567343980173No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAGATCGGAAGAGCAC26900.11874948405777142No Hit
ATATCTTCAGGAGATCGTCATGGACAGATTCCCACGAGATCGGAAGAGCA25920.11442329467574108No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA25520.11265750309123888No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG25060.11062684276906139No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG24930.11005296050409817Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA24360.10753670749618259Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)