FastQCFastQC Report
Fri 14 Jul 2023
SRR9691720_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691720_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936490
Total Bases147.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC1216546.282190974391812No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG968455.001058616362594No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC426242.2010957970348413No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG392502.0268630356986095No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG370101.9111898331517334No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA280201.446947828287262No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA170920.8826278472907166No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA169910.8774122252115941No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA149660.7728415845163156No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC137900.7121131531792056Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG124840.6446715449085717No Hit
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA119330.6162180026749428No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC118100.6098663044993777No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA110890.572633992429602No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA96880.5002866010152389Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC92280.4765322826350769No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC82320.42509901935976946TruSeq Adapter, Index 6 (100% over 35bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG76410.39457988422351775No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA72010.3718583622946671No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA62910.3248661237599988No Hit
ACGGTCCTAAGGTAGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC62800.3242980857117775Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA52280.2699729923727982No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAAG49540.25582368098983216No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA48790.2519506942974144No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT48140.2485941058306524No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA47730.246476873105464No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG47650.24606375452493945No Hit
ACGGTCCTAAGGTAGCGAAAAGATCGGAAGAGCACACGTCTGAACTCCAG47080.243120284638702Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA46720.24126125102634147No Hit
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGG45920.23713006522109592No Hit
ACGGTCCTAAGGTAGCGAAAGATCGGAAGAGCACACGTCTGAACTCCAGT45370.23428987497998957Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG44820.23144968473888325No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA44450.22953901130395715No Hit
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC40260.20790192564898347TruSeq Adapter, Index 6 (100% over 36bp)
ACGGTCCTAAGGTAGCGAAATTCCAGATCGGAAGAGCACACGTCTGAACT40200.20759208671359006Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA37870.1955600080558123No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC36170.18678123821966547Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA32640.16855238085401938Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG31980.16514415256469178No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG31640.16338839859746243No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG30200.15595226414802038Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCAAGATCGGAAGAGCACACGTCTGAAC28080.14500462176411963Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG27960.14438494389333278No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA27900.14407510495793938No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG27850.14381690584511153Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT27680.14293902886149684No Hit
AAGGCTGAGGCGTGATGACGAGAGATCGGAAGAGCACACGTCTGAACTCC26870.13875620323368568Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAGACTCTGAACGCTAAAGTTGAGATCGGAAGAGCACACGTCTGAACT25250.1303905519780634Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA24250.12522656972150645No Hit
AAGGCTGAGGCGTGATGACGAGGCAGATCGGAAGAGCACACGTCTGAACT24140.12465853167328517Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATAGATCGGAA23770.1227478582383591No Hit
AAGGCTGAGGCGTGATGACGAGATCGGAAGAGCACACGTCTGAACTCCAG23700.1223863794804001Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTCC21070.10880510614565528Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG20650.10663623359790136No Hit
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA20100.10379604335679503Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT20010.1033312849537049No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG19720.10183373009930337No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)