FastQCFastQC Report
Fri 14 Jul 2023
SRR9691721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817811
Total Bases138.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC1120166.1621367677937915No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG890674.89968429061107No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC391062.15126875126182No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG350841.9300136262790795No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG336171.8493121672165036No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA258021.4193994865252768No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA158080.869617358460258No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA154660.850803521378185No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA136110.748757709134778No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC131490.7233425257081182Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG114650.6307036320057475No Hit
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA107690.5924158232071431No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC107060.588950116376235No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA101450.5580888222152908No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA89410.4918553138912681Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC85120.46825550070936967No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC77700.42743717581200685TruSeq Adapter, Index 6 (100% over 35bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG70110.3856836601824942No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA64050.35234686114233No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA58530.32198066795722985No Hit
ACGGTCCTAAGGTAGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC57670.31724970307694256Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA45790.2518963742655314No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT44660.24568010645771204No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG44310.24375471377387417No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAAG44280.2435896801152595No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA44230.24331462401756837No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA42960.23632819913621383No Hit
ACGGTCCTAAGGTAGCGAAAAGATCGGAAGAGCACACGTCTGAACTCCAG42890.23594312059944628Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGTCCTAAGGTAGCGAAAGATCGGAAGAGCACACGTCTGAACTCCAGT42780.2353379971845258Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA42560.23412775035468483No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA41730.22956181913301216No Hit
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGG40890.2249408766918013No Hit
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG40830.22461080937457195No Hit
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC38030.209207667903869TruSeq Adapter, Index 6 (100% over 36bp)
ACGGTCCTAAGGTAGCGAAATTCCAGATCGGAAGAGCACACGTCTGAACT36500.2007909513145206Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC35070.19292434692055443Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA34950.19226421228609575No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA31630.1740004873994051Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG28790.15837730105054926No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG28370.15606682982994383No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG28220.15524166153687044Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGCTGAGGCGTGATGACGAGGCAAGATCGGAAGAGCACACGTCTGAAC26830.1475951020210572Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT26310.1447345186050695No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG25900.14247905860400228Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGGCTGAGGCGTGATGACGAGAGATCGGAAGAGCACACGTCTGAACTCC25500.14027860982247328Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG24870.13681290299156515No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA24740.1360977571375682No Hit
TTCAGACTCTGAACGCTAAAGTTGAGATCGGAAGAGCACACGTCTGAACT24480.13466746542957436Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGCTGAGGCGTGATGACGAGGCAGATCGGAAGAGCACACGTCTGAACT23100.12707591713329933Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATAGATCGGAA23090.1270209059137611No Hit
AAGGCTGAGGCGTGATGACGAGATCGGAAGAGCACACGTCTGAACTCCAG21490.11821911078764515Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA21480.11816409956810692No Hit
AGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTCC19780.10881219224660868Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG19530.10743691175815308No Hit
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA19360.10650172102600326Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT18860.103751160049092No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18560.10210082346294527No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC18400.10122064395033369Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)