FastQCFastQC Report
Fri 14 Jul 2023
SRR9691724_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691724_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2824304
Total Bases214.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG596852.1132640112395835No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG254310.9004342308759964No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC244560.865912451350846No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA183320.6490802689795433No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC159440.5645284643579445TruSeq Adapter, Index 5 (100% over 35bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG124870.44212662659543733No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC95570.3383842532531909No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG92410.32719565599170625No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA86570.30651799522997525No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA79680.2821226043655357No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA70470.2495128003217784Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA69960.2477070457004628Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA69440.2458658841257882No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA66730.23627059976546436No Hit
TTCTTGGACTGGACCATAAAATTTCAGATCGGAAGAGCACACGTCTGAAC59160.2094675360726041Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA57650.20412108611537566No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG46200.16358012451917356Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA44990.15929588316271903No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA44340.15699443119437567Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG43240.15309966632487154No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA42110.14909868059529002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA41890.1483197276213892TruSeq Adapter, Index 5 (100% over 36bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT41590.14725751902061535No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC41580.14722211206725622Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG40640.14389385845149813No Hit
CGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA39440.13964502404840273TruSeq Adapter, Index 5 (100% over 36bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC38940.1378746763804463Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGT38440.13610432871248987Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC38260.13546700355202557Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA35020.12399515066366794Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC34900.12357026722335838Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC34210.12112718744157853Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGAGTATGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA32320.11443527325670323Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC32310.11439986630334412No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA31410.11121324050102255Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC29890.10583138359043502No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA29780.10544190710348463No Hit
ATTCTTGGACTGGACCATAAAATTTCAGATCGGAAGAGCACACGTCTGAA29750.10533568624340722Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC29500.10445051240942903Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCTG29130.10314045513514125Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)