FastQCFastQC Report
Fri 14 Jul 2023
SRR9691725_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691725_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2752852
Total Bases209.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG567922.063024092831725No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG242320.8802507363272708No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC232210.8435251876962511No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA171520.6230629180210197No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC154560.5614540847092397TruSeq Adapter, Index 5 (100% over 35bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG120790.43878130753124395No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC91920.3339082522416752No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG88080.31995908243523447No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA83860.3046295260333647No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA74890.272045137188632No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA67520.2452729024299163Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA66010.2397876820112378Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA63940.23226820766245335No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA62820.22819969980224145No Hit
TTCTTGGACTGGACCATAAAATTTCAGATCGGAAGAGCACACGTCTGAAC57960.2105452817659649Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA56090.20375232667793255No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG45670.165900673192747Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44010.15987056332850438No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA42640.15489390639235237Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA41740.15162456971896782No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA41020.14900910038026016Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG40230.1461393493002893No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT39810.14461365885270983No Hit
CGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA39240.1425430789595663TruSeq Adapter, Index 5 (100% over 36bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC39240.1425430789595663Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA38590.1401818913621219TruSeq Adapter, Index 5 (100% over 36bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG36510.13262609105029985No Hit
TCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGT36380.13215385353081097Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC35960.1306281630832315Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC35400.12859390915312555Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA33330.1210744348043411Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC33260.12082015306307786Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC31920.11595247401603864Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC30790.11184764019278916No Hit
AGGGAGTATGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA30600.11115744689507465Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA30550.11097581707988662Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC28760.1044734696961551No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC28660.10411021006577906Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA28580.10381960236147822No Hit
ATTCTTGGACTGGACCATAAAATTTCAGATCGGAAGAGCACACGTCTGAA28290.10276614943338763Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)