FastQCFastQC Report
Fri 14 Jul 2023
SRR9691726_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691726_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793043
Total Bases212.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG590282.1133938861664503No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG252210.9029936166396293No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC242820.8693743705342167No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA179680.643312687989408No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC157760.5648319771661231TruSeq Adapter, Index 5 (100% over 35bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG123560.4423848827246841No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC93630.3352257734664307No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG92990.33293436585115227No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA86630.3101635026743233No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA77280.2766874695448656No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA69630.24929798789349109Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA68210.24421392724709215No Hit
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA67710.2424237650476559Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA67440.2414570774599603No Hit
TTCTTGGACTGGACCATAAAATTTCAGATCGGAAGAGCACACGTCTGAAC58410.2091267481381418Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA57870.20719337296275067No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG47110.16866908243088274Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA44910.16079236875336328No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA43230.15477742376325748Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG42310.1514835253162948No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA42170.15098227990045265Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA41280.14779579118545616TruSeq Adapter, Index 5 (100% over 36bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT40790.14604143223000862No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC40300.1442870732745611Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG39740.1422820916111925No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC38150.13658937581698527Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA37910.13573009796125587TruSeq Adapter, Index 5 (100% over 36bp)
TCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGT37710.13501403308148138Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC36710.1314337086826089Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC34710.12427305988486394Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA34120.12216066848952917Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC33980.12165942307368702Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGAGTATGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA32350.11582349430352486Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC31800.113854315884145No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA31740.11363949642021265Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC30530.10930730389757694Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA30460.10905668118965588No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC30220.10819740333392648No Hit
AACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCTG28690.10271950700365157Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCTTGGACTGGACCATAAAATTTCAGATCGGAAGAGCACACGTCTGAA28270.10121577075612512Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)