FastQCFastQC Report
Fri 14 Jul 2023
SRR9691727_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691727_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2588029
Total Bases196.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG1284564.9634683382605065No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG1127374.356094927838908No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC880493.4021643497812426No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC475441.837073695851167No Hit
ACGGTCCTAAGGTAGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC358371.3847217322526137Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGTCCTAAGGTAGCGAAAAGATCGGAAGAGCACACGTCTGAACTCCAG324171.2525748359079438Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG312861.2088736254501011No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA304451.1763778535711926No Hit
ACGGTCCTAAGGTAGCGAAAGATCGGAAGAGCACACGTCTGAACTCCAGT290791.1235963739200758Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG197330.7624721361313958No Hit
ACGGTCCTAAGGTAGCGAAATTCCAGATCGGAAGAGCACACGTCTGAACT192470.7436933666508373Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA183810.710231608687538No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAAG164460.635464285755685No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA141560.546979960425482No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA131100.5065631026545684No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA128140.4951258274153806No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT122830.4746082829829187No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC122320.4726376713707613No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG117290.45320203135281717No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA115330.4456287004511928No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG111460.4306752358648222TruSeq Adapter, Index 4 (100% over 35bp)
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA94390.3647177060226141No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA84960.32828071091939076No Hit
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA84860.32789431648563444No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT77060.29775555065263953No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC65070.2514268580452537No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT64850.2505767902909898No Hit
ACGGTCCTAAGGTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA64320.24852889979208115Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA58090.22445652656906084No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA57340.22155856831588827Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA54200.209425783095939No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA53180.20548455987162434No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC53100.2051754443246192Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA52970.20467313156073597No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG51880.20046143223279184No Hit
ACGGTCCTAAGGTAGCGAAATTAGATCGGAAGAGCACACGTCTGAACTCC50220.19404728463243648Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA47790.1846578998921573Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA46350.17909382004606594Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT45890.17731640565078677Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG42540.1643721921199492No Hit
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGG41260.15942634336786798No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40410.15614199068093904No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG39110.15111886304210656Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCAGATCGGAAGAG36640.14157492052832482No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATAGATCGGAA36470.14091804999093904No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA35960.1389474383787817Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG35320.13647451400274108No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGGAGATCGGAAG35310.13643587455936546No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC35080.13554716736172587Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC34910.13489029682434006Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA34340.1326878485519289No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA32650.12615778262144667No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG32210.12445764711291876No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC32100.12403261323578677Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGG31940.12341438214177661TruSeq Adapter, Index 10 (97% over 36bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA31900.12325982436827408No Hit
TCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGT30680.11854581227644667Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT30400.11746390786192891No Hit
ACGGTCCTAAGGTAGCGAAATTCTAGATCGGAAGAGCACACGTCTGAACT29980.11584105124015226Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC29600.11437275239187814Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG29460.11383180018461926No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG29030.11217030411946698No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA27010.10436513655758879No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC26890.1039014632370812No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGTAGATCG26860.1037855449069543No Hit
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG26660.1030127560394416No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)