FastQCFastQC Report
Fri 14 Jul 2023
SRR9691729_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691729_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2491748
Total Bases189.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG1243134.988987650436561No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG1098634.409073469708815No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC854523.429399762736842No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC462431.8558457757365512No Hit
ACGGTCCTAAGGTAGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC346581.3909111194229913Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGTCCTAAGGTAGCGAAAAGATCGGAAGAGCACACGTCTGAACTCCAG311231.2490428406082799Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG303131.2165355405121223No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA297031.192054734266868No Hit
ACGGTCCTAAGGTAGCGAAAGATCGGAAGAGCACACGTCTGAACTCCAGT279741.1226656949258111Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG194030.7786903009453604No Hit
ACGGTCCTAAGGTAGCGAAATTCCAGATCGGAAGAGCACACGTCTGAACT184660.7410861772538796Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA179850.7217824595424577No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAAG155140.6226151280145504No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA134220.5386580023341043No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA127180.5104047439789257No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA125420.5033414293901309No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT122720.4925056626914118No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC120550.48379691686318194No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG114500.4595167729642002No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA111390.4470355750260459No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG110360.44290193069283096TruSeq Adapter, Index 4 (100% over 35bp)
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA91470.3670916962710515No Hit
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA82560.3313336661652783No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA80120.3215413436671766No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT75540.30316067274860864No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT63490.25480104729691766No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC62390.250386475678921No Hit
ACGGTCCTAAGGTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA60570.24308236627459923Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA57750.23176500994482588No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA54460.21856142756009034Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA51650.20728420369957157No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA51520.20676248159926286No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA50890.2042341360362284No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC50760.20371241393591968Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG50250.2016656580039394No Hit
ACGGTCCTAAGGTAGCGAAATTAGATCGGAAGAGCACACGTCTGAACTCC48750.19564578761576212Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT46270.18569293524064232Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA44950.1803954492990463Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA44840.17995399213724664Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGG40490.16249636801153247No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG40120.16101146664911542No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG37480.15041649476592336Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA35760.14351371005414673Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCAGATCGGAAGAG35200.1412662917758939No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATAGATCGGAA34700.1392596683131681No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC34500.1384570189280778Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC34340.13781489942000555Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGGAGATCGGAAG32870.1319154264395918No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG32820.13171476409331923No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA32290.1295877432228299No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC32200.12922655099953928Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG32110.12886535877624863No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31050.12461131703527001No Hit
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGG30630.12292575332658037TruSeq Adapter, Index 10 (97% over 36bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA30420.12208297147223557No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA30360.12184217665670846No Hit
TCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGT29990.1203572752942914Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT29540.11855131417783821No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC29210.1172269426924392Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG28970.11626376343033085No Hit
ACGGTCCTAAGGTAGCGAAATTCTAGATCGGAAGAGCACACGTCTGAACT28510.11441766984462313Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG28290.1135347555210238No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC27460.11020376057289903No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA26440.10611024870893847No Hit
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG25820.10362203561515852No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGTAGATCG25610.10277925376081369No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)