FastQCFastQC Report
Fri 14 Jul 2023
SRR9691730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632040
Total Bases200 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG1362135.175187307183782No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG1181494.488875549003814No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC912013.465030926581663No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC492801.872311970942691No Hit
ACGGTCCTAAGGTAGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC379691.4425692618653212Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGTCCTAAGGTAGCGAAAAGATCGGAAGAGCACACGTCTGAACTCCAG336761.2794638379355936Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG329391.251462743727299No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA323141.2277169039984195No Hit
ACGGTCCTAAGGTAGCGAAAGATCGGAAGAGCACACGTCTGAACTCCAGT308251.171144815428337Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG206050.7828528441816994No Hit
ACGGTCCTAAGGTAGCGAAATTCCAGATCGGAAGAGCACACGTCTGAACT204120.7755201288734214Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA190970.7255588820838589No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAAG173830.6604382912113798No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA147260.5594899773559673No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA141670.5382516983024574No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA133570.5074770900138296No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC131180.4983966809015061No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT129360.4914818923724563No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG124000.4711174602209693No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA118920.45181684168933606No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG115210.43772131122627317TruSeq Adapter, Index 4 (100% over 35bp)
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA96620.36709168553669397No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA89090.33848269783133994No Hit
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA88050.3345313901004544No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT80350.3052765155544749No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC67750.25740490266105376No Hit
ACGGTCCTAAGGTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA67610.2568729958511269Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT65770.24988222063494475No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA61080.23206334250239358No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA57010.21660005167094726No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA56280.21382653759061412No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA56270.2137885442470479Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA54560.20729168249722646No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC54390.206645795656601Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG53790.20436619504262854No Hit
ACGGTCCTAAGGTAGCGAAATTAGATCGGAAGAGCACACGTCTGAACTCC53090.20170666099299403Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT49110.18658531025364358Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA48970.18605340344371665Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA47740.1813802221850732Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG44040.16732268506557652No Hit
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGG43100.16375131077035304No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG40460.1537210680688743Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATAGATCGGAA37920.1440707588030577No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCAGATCGGAAGAG37480.14239905168614458No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA37180.14125925137915837Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC37120.14103129131776113Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGGAGATCGGAAG36340.138067810519597No Hit
AATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGG35980.1367000501512135No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC35950.13658607012051488Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA35080.13328064923025484No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA34680.13176091548760657No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG33810.12845549459734654No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA33640.12780960775672104No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC33380.12682178082399964Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGG32900.12499810033282169TruSeq Adapter, Index 10 (97% over 36bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT31680.12036291241774441No Hit
TCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGT31330.11903314539292716Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG31200.11853923192656646No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC30780.11694351149678577Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGTCCTAAGGTAGCGAAATTCTAGATCGGAAGAGCACACGTCTGAACT30710.11667755809182231Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG30610.11629762465616023No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC28680.10896490934788226No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA28300.10752116229236638No Hit
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG27720.10531754836552636No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGTAGATCG27330.10383580796644429No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG27240.10349386787434842No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC26690.10140423397820703Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)