FastQCFastQC Report
Fri 14 Jul 2023
SRR9691732_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691732_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485953
Total Bases188.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG2195878.833111486822157No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC679532.733478871080829No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG324111.3037655981428449No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG256801.0330042442475782No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC234810.9445472219305836Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA223530.8991722691458769No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA216800.8721001563585474No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA199680.8032332067420421No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA155260.6245492171412734No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA147620.5938165363544685TruSeq Adapter, Index 7 (100% over 35bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC118120.4751497715363083Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC112180.4512555144847871TruSeq Adapter, Index 7 (97% over 35bp)
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG111880.4500487338256194No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA101070.40656440407360883No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC96110.386612297175369No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG90250.36303984829962593No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG84080.33822039274274296No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA81670.3285259214474288No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA79150.3183889639104198No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG78430.3154926903284173No Hit
AAGGCTGAGGCGTGATGACGAGGCAGATCGGAAGAGCACACGTCTGAACT73850.2970691722651233Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC70410.2832314207066666Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC68130.27405988769699186Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA66360.26693988180790224No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC66060.2657331011487345No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA62770.2524987399198617Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGCTGAGGCGTGATGACGAGAGATCGGAAGAGCACACGTCTGAACTCC59440.23910347460309989Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG58780.2364485571529309No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA58130.23383386572473414No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG53930.2169389364963859Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA53730.21613441605694075No Hit
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGG52720.21207158783774271No Hit
AAGGCTGAGGCGTGATGACGAGATCGGAAGAGCACACGTCTGAACTCCAG50660.2037850273114576Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTCC50340.20249779460834536Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA47040.18922320735750034No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA41880.16846658001981532No Hit
ATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCC41760.16798386775614826Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAAG40820.16420262169075603No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT40750.16392103953695022Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA38180.15358295189007998No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36030.14493435716604455No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC35580.14312418617729297No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA35300.14199785756206976No Hit
AAGGCTGAGGCGTGATGACGAGGCAAGATCGGAAGAGCACACGTCTGAAC34470.13865909773837237Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCATGGGTCGGGACAGTGGCCAATCCAGATCGGAAGAGCACACGTCTGAA31840.12807965395966858Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC30560.1229307231472196No Hit
AAGGCTGAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC30400.12228710679566349TruSeq Adapter, Index 7 (97% over 35bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA30270.12176416851002413Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC29690.11943105923563319TruSeq Adapter, Index 7 (97% over 35bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG29240.11762088824688159No Hit
AGCTGTCTTCTGACGTTCAGACTCAGATCGGAAGAGCACACGTCTGAACT28560.11488551875276805Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA28460.11448325853304549Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA27700.11142608086315388No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGAAC27640.11118472473132034Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGCTGAGGCGTGATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC27610.11106404666540357Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGA27450.11042043031384745No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATAGATCGGAA27120.10909297158876294No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAAGAGC26840.10796664297353972No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG25870.10406471884223073No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAA25860.10402449282025847No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA25740.10354178055659137No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA24940.10032369879881076No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)