FastQCFastQC Report
Fri 14 Jul 2023
SRR9691734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538621
Total Bases192.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG2310849.102737273504001No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC712282.8057752614510005No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG348941.3745257760020106No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG263431.0376893596956773No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC245180.9657999362646098Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA235240.9266448201602366No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA225150.8868988320824573No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA207770.8184364660971449No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA161940.6379053824891545No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA153870.6061164703199099TruSeq Adapter, Index 7 (100% over 35bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC124880.4919206135929704Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC119720.4715946177078028TruSeq Adapter, Index 7 (97% over 35bp)
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG114910.452647323093916No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA107650.42404911958106395No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC100890.3974204893128986No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG91640.3609833842861932No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG90040.3546807499031955No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA85810.3380181602531453No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA84970.3347092772020715No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG81880.3225373145499072No Hit
AAGGCTGAGGCGTGATGACGAGGCAGATCGGAAGAGCACACGTCTGAACT77600.30567776757538834Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC75420.29709042822855397Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC72100.2840124618838338Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA71730.28255497768276555No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC69770.2748342505635934No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA65000.2560445218092815Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA61680.24296655546456125No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGG60670.23898801751029397No Hit
AAGGCTGAGGCGTGATGACGAGAGATCGGAAGAGCACACGTCTGAACTCC59680.23508826248581416Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG56770.22362534620173707Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA56720.2234283888772684No Hit
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGG55150.21724392888895191No Hit
AAGGCTGAGGCGTGATGACGAGATCGGAAGAGCACACGTCTGAACTCCAG54460.21452591781128413Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTCC52060.2050719662367876Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA48130.1895911205335495No Hit
ATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCC43990.17328305406754296Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA43550.1715498296122186No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT42830.16871364413986964Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAAG42060.165680501343052No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA40230.15847186326749838No Hit
AAGGCTGAGGCGTGATGACGAGGCAAGATCGGAAGAGCACACGTCTGAAC37810.14893912876321436Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC36860.14519693959830948No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA36130.1423213626610668No Hit
TCATGGGTCGGGACAGTGGCCAATCCAGATCGGAAGAGCACACGTCTGAA32990.1299524426844338Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC32320.1273132145365535No Hit
CGGACGTGGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC31510.12412250588016092TruSeq Adapter, Index 7 (97% over 35bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG31500.1240831144152672No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA31030.1222317155652616Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGCTGAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC30460.11998640206631868TruSeq Adapter, Index 7 (97% over 35bp)
AGCTGTCTTCTGACGTTCAGACTCAGATCGGAAGAGCACACGTCTGAACT30390.11971066181206252Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA29610.11663812755035115Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGAAC28870.11372315914821472Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGA28650.11285654692055254No Hit
AAGGCTGAGGCGTGATGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC28210.11112332246522817Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATAGATCGGAA28210.11112332246522817No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAA28100.1106900163513971No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAAGAGC28020.11037488463224718No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA27950.11009914437799104No Hit
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG27210.1071841759758546No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA27120.10682965279181099No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA26970.10623878081840496No Hit
ATCCGAATTCGAGGCCGGCGACTAGTCTTGGACTCCAGATCGGAAGAGCA26490.10434799050350564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)