FastQCFastQC Report
Fri 14 Jul 2023
SRR9691735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605867
Total Bases198 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG1744666.6951229667515655No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC653752.5087619590715873No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC431081.6542670827022254No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG300531.1532821897664003No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG293551.1264964789070202No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG246030.9441387453772583No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA245190.9409152500875909No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA212690.816196682332598No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC185740.7127762084557654No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG159870.6135002285227911No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA131040.5028652651881312No Hit
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA130870.5022128911414128No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA130680.5014837672068452No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA126120.48398479277722156No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA114900.4409281056938056No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC109420.4198986364231176Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA102430.3930745506198129Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA101690.39023480476939154No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG99220.38075619362001206No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT99100.3802956942929167TruSeq Adapter, Index 1 (100% over 35bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC85800.32925701887318115No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC84580.3245752757143784Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT81400.3123720435463514Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT78030.2994396874437567No Hit
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT70620.2710038539956184TruSeq Adapter, Index 27 (100% over 36bp)
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA67970.2608344938555959Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT67620.25949137081823437No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC67010.257150499238833Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGG65660.2519698818090102No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC64120.24606014044461977Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC63990.24556126617359977Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA63050.24195402144468617Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA60830.23343478389342204Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAAG54570.20941206899661416No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC51300.19686346233326568Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCATGGGTCGGGACAGTGGCCAATCCAGATCGGAAGAGCACACGTCTGAA49960.1917212198473675Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG45380.17414549552989467No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG43860.16831250405335346No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG43580.16723800562346428No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGTAGATCG43430.16666238146459508No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA43330.16627863202534895No Hit
AGGGAGTATGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA43060.1652425085393844Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA41520.15933276717499395No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA40950.15714539537129102No Hit
ATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCC40210.15430564952086964Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA38600.14812728354900692No Hit
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG35590.13657642542769832No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA34480.1323168066520663No Hit
CGGACGTGGGGGTTCAAGTCCCCCAGATCGGAAGAGCACACGTCTGAACT34470.13227843170814166Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA34160.13108880844647866Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC33000.12663731495122355Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA31550.12107294808215462No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGAAC30900.11857857672705477Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGAA30680.11773432796071327Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAGATCGGAAGAGCACACGT29820.11443408278319653No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA29580.11351308412900582No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG28920.11098033782998135Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA28470.10925346535337375No Hit
ACGGATCCGAATTCGAGGCCGGCGACTAGTCTTGGACTCCAGATCGGAAG28260.10844759153095687No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA27450.10533922107306322No Hit
TTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAA26780.10276809983011412No Hit
ATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAACTCCA26230.1006574779142604Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGCTGAGGCGTGATGACGAGATCGGAAGAGCACACGTCTGAACTCCAG26140.10031210341893888Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)