FastQCFastQC Report
Fri 14 Jul 2023
SRR9691738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2640303
Total Bases200.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG1766296.689724626302359No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC659922.499410105582579No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC442411.6756031410031351No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG302571.145966959095225No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG297401.1263858731365302No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG254400.963525777155122No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA249090.9434144490234643No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA214260.8114977712785237No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC190420.7212051041111569No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG160050.6061804270191716No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA134150.5080856250210677No Hit
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGA132850.5031619477006994No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA132330.5011924767725522No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA125590.4756651035884896No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA113280.4290416668086958No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC112640.42661770258943765Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA104880.3972271364309323Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA101680.3851073153346415No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT100180.3794261491957552TruSeq Adapter, Index 1 (100% over 35bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG98760.37404797858427613No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC88170.3339389456437386No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC84990.32189487342929957Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT80190.30371514178486336Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT79510.30113967980190154No Hit
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT71850.2721278580526553TruSeq Adapter, Index 27 (100% over 36bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT70850.2683404139600644No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCAGATCGGAAGAGCACACGTCTGAA68180.2582279382328468Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGG67640.2561827184228477No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC67520.25572822513173676Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC67050.25394812640821907Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC64380.24383565068100138Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA64220.24322965962618684Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA61260.2320188251121178Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAAG55150.2088775417063875No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC52180.19762883275139254Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCATGGGTCGGGACAGTGGCCAATCCAGATCGGAAGAGCACACGTCTGAA50540.1914174244395435Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGAAG47420.17960059887065993No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGTAGATCG45290.17153334295344133No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG45220.17126822186695997No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG43780.1658143023736291No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA42910.16251922601307503No Hit
AGGGAGTATGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA42900.1624813515721491Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA41940.15884540524326185No Hit
ATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCC41140.15581544996918914Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA40880.1548307145051155No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA39290.148808678397896No Hit
TGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGGAAGAGCACACG35680.13513600522364289No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGA34780.1317273055403111No Hit
CGGACGTGGGGGTTCAAGTCCCCCAGATCGGAAGAGCACACGTCTGAACT34420.13036382566697838Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC34370.13017445346234885Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA33620.12733387039290567Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGAAC32090.12153908093124162Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA31240.11831975345253935No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGAGATCGGAAGAGCACACGTCTG29470.11161597740865348Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAGATCGGAAGAGCACACGT29460.11157810296772758No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA29450.11154022852680166No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGAA29090.11017674865346894Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAA28890.10941925983495077No Hit
ACGGATCCGAATTCGAGGCCGGCGACTAGTCTTGGACTCCAGATCGGAAG27460.1040032147825458No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA27230.10313210264124989No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT26520.10044301733551037No Hit
AAGGCTGAGGCGTGATGACGAGATCGGAAGAGCACACGTCTGAACTCCAG26490.10032939401273264Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)