FastQCFastQC Report
Fri 14 Jul 2023
SRR9691739_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691739_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3723299
Total Bases282.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTC506141.3593858564676111Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA468421.2580778497778449Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC338560.909301133215463No Hit
AATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACT318690.8559344817593215Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCC292880.78661423646073Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG286810.7703114898910886No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG253000.6795049229191639No Hit
ATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCC238060.6393792171942141Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA227330.6105606882498558No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC221390.5946070944074059Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA180460.4846777011462147No Hit
ATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACT166180.4463246169593148Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC163260.4384821095485482No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC158870.42669149053030664No Hit
ATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAA149110.40047817808884006Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA147590.3963957769709067No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG147340.395724329418615No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC142380.38240280998114845No Hit
AATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGA140500.37735352438791514Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA139390.37437229725574017No Hit
ATTCGGACTATGCCAGGCAAGAAATCCAGATCGGAAGAGCACACGTCTGA133420.3583381297070152Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA131010.35186537530292356No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG129640.34818584271636527No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT122190.32817670565807366No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA121480.32626979460956534No Hit
AATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAAC118270.3176484080381404Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAACTCCA117890.316627807758657Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA114710.30808699489350705No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA109630.29444318063094044Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTC100630.27027106874844053Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG99380.26691383098698224No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC97670.26232112972930727No Hit
TGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTCCA78610.2111299683425908Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG78500.2108345314195825No Hit
ATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC78000.20949163631499915Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCTGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGA75190.20194456582724082Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAGCGTTCTGACCTGTAGCAATCGAGATCGGAAGAGCACACGTCTGAAC72480.1946660743603992Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA72130.19372604778719088Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACT66520.17865876471376593Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCGGACTATGCCAGGCAAGAAATAGATCGGAAGAGCACACGTCTGAAC65230.17519409534394095Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA64820.17409292135818263No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC57390.15413750010407437Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT55610.1493567935317577No Hit
ATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT55320.14857791437109938Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG55170.1481750458397244No Hit
TGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAACTC53410.14344805507159109Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC52330.1405474016456911No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC52310.14049368584150776No Hit
TCTGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAA49370.1325974626265578Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGA48810.13109342010942446Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG46690.12539954486599114No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC45930.12335834430702448Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT45490.12217659661499117Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC45320.12172001227943284No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTAGATCGGAAGAGCACAC45170.12131714374805783No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC44180.11865821144098285Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA44130.11852392193052452No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG44070.11836277451797451Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA43410.11659015297992453No Hit
ATCTTGTCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGATCGGA42430.1139580785749412No Hit
AAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCAC42230.11342092053310787No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA42000.11280318878499954No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT41950.11266889927454121No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT39080.1049606813742329Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA38480.10334920724873291No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA38270.10278519130480791Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA37420.10050226962701625No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT37360.10034112221446626Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)