FastQCFastQC Report
Fri 14 Jul 2023
SRR9691740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3868748
Total Bases294 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTC515051.3313092504345074Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA474631.2268310057930887Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC348600.9010667016823013No Hit
AATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACT319040.8246595539435497Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG299400.7738937764878974No Hit
TCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCC297590.7692152603374528Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG265090.6852087548736697No Hit
ATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCC244350.6315996803100125Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA233570.6037353686515637No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC224780.5810148399430514Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA182500.4717288383735513No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC168590.4357740540350522No Hit
ATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACT168590.4357740540350522Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC166590.4306044229295886No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA152970.3953992351013816No Hit
ATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAA151510.3916254043943932Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG149230.38573202493416475No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC146560.37883056740837084No Hit
AATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGA145090.37503088854585515Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA143040.369732016662755No Hit
ATTCGGACTATGCCAGGCAAGAAATCCAGATCGGAAGAGCACACGTCTGA136830.35368031208029055Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG136550.3529565637255257No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA134700.34817465495297184No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT130260.3366980738988427No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA122130.3156835234551333No Hit
ATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAACTCCA120290.3109274628381068Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAAC119600.30914394010672186Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA119080.30779983601930133No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA109800.28381274768995035Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG104800.2708886699262914No Hit
TCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTC103070.2664169390200654Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC101530.26243632306885845No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG84070.21730544351816145No Hit
ATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC82110.21223920503480712Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTCCA80350.20768992966199917Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA76120.1967561598739437Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGA75340.19474000374281292Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAGCGTTCTGACCTGTAGCAATCGAGATCGGAAGAGCACACGTCTGAAC71730.18540881959745117Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA68630.17739589138398262No Hit
TGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACT67010.17320849018855713Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCGGACTATGCCAGGCAAGAAATAGATCGGAAGAGCACACGTCTGAAC64600.16697908470647352Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC60100.15534741471918048Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT57750.14927309817026077No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC57520.14867859059313246No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG57150.1477222088386217No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC55160.14257842588868544No Hit
ATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT55060.14231994433341227Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAACTC53200.13751218740533114Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG50960.13172220056721193No Hit
TTGAATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGA49540.1280517624823328Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAA49210.1271987733499313Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA48120.12438132439745364No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTAGATCGGAAGAGCACAC47380.12246856088843214No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG46400.11993544164675499Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT46320.11972865640253642Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA45870.11856548940380712No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC45710.11815191891537002Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT45280.11704044822769537No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC44510.11505014025209188No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC44190.11422299927521772Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA42930.11096613167877567No Hit
AAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCAC42930.11096613167877567No Hit
ATCTTGTCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGATCGGA42570.11003559807979221No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA42060.10871734214789901No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT40530.10476257435221939Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA39970.10331507764268957Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA39820.10292735530977981No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)