FastQCFastQC Report
Fri 14 Jul 2023
SRR9691741_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691741_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3908649
Total Bases297 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTC526881.3479849431350832Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA482731.2350303135431193Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC354950.9081142870592883No Hit
AATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACT330780.8462770640187953Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCC302530.7740014516524762Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG301610.7716476971966529No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG275320.7043866051927405No Hit
ATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCC251880.6444170351443683Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA238660.6105946069856873No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC226710.5800213833475454Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA187580.4799100661123575No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC171690.43925663317427577No Hit
ATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACT169480.4336025056227868Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC166540.42608072507917694No Hit
ATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAA154490.39525165856540195Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA153150.39182336403192No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG150610.3853249549908421No Hit
AATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGA148620.3802336817657456Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC146340.3744004642013135No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA145090.3712024282559012No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG138680.3548028999278267No Hit
ATTCGGACTATGCCAGGCAAGAAATCCAGATCGGAAGAGCACACGTCTGA137610.3520653811585538Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT136020.3479974794359893No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA135340.34625774788168495No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA124930.31962450452829094No Hit
ATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAACTCCA122680.31386803982654876Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAAC121980.31207713969711787Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA118630.3035064033634128No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA108640.2779477000876774Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG106240.27180747107248565No Hit
TCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTC104950.26850709797682015Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC102300.26172726177254596No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG85720.21930851299259668No Hit
TGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTCCA83470.21355204829085445Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC81640.20887012366677082Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA76470.19564304699654536Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGA76240.19505460838258948Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAGCGTTCTGACCTGTAGCAATCGAGATCGGAAGAGCACACGTCTGAAC73810.1888376265047079Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACT68630.17558496554691916Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA66900.17115888379846847No Hit
ATTCGGACTATGCCAGGCAAGAAATAGATCGGAAGAGCACACGTCTGAAC66480.1700843437208099Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC60720.15534779408434984Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT59260.15161248810010824No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG57240.14644446201232192No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC56760.14521641620928355No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC56670.14498615762121386No Hit
ATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT56550.1446791461704543Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAACTC54310.1389482657562754Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG53120.13590373553624283No Hit
TCTGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAA49820.12746092064035425Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGA49700.12715390918959468Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA49070.12554209907310684No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT47980.12275341172870728No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC47620.12183237737642853Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT47330.12109043303709285Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA47220.12080900587389658No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG47000.12024615154750402Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTAGATCGGAAGAGCACAC46720.11952979149573165No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC45290.11587123837417992Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC45130.11546188977316714No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA43940.1124173595531346No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA43720.11185450522674203No Hit
AAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCAC42880.10970542507142493No Hit
ATCTTGTCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGATCGGA42550.10886114358183607No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA41310.10568869192398704No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT41000.10489557900952477Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA39790.10179988021436562Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)