FastQCFastQC Report
Fri 14 Jul 2023
SRR9691743_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691743_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4787587
Total Bases363.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTC1022542.1358149731795995Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACT601701.25679178258275Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCC579221.2098370222828325Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA475580.9933605384090148Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG383670.8013849147806609No Hit
ATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCC332390.6942745896836966Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA297930.6222967854161188No Hit
ATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACT284930.5951432318618961Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC271940.5680105656565615No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG255970.5346534694826434No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA248620.5193012680500636No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC243960.5095677634683192Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAA211630.4420389645138563Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC209330.4372348742696477No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG208760.43604429538303946No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC193140.40341825642019663No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA189280.3953557397494813No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA182240.3806510461324254No Hit
AATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAAC181610.37933514315248995Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTC179410.37473992639716003Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCGGACTATGCCAGGCAAGAAATCCAGATCGGAAGAGCACACGTCTGA178840.3735493475105518Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA178280.3723796559728314No Hit
ATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAACTCCA176100.36782621391527714Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA173030.3614137978067031No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC165620.34593627228079615No Hit
AATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGA158610.3312942407104038Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC154570.32285575175970693No Hit
TGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTCCA150870.3151274326711974Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC142110.29683011504542894Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA123060.2570397154140489Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG122570.25601623531854356No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG120600.25190142758763445No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA106650.22276357588906479Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG104240.21772972480708966No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA102740.21459662247391015No Hit
TGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACT100940.2108368996740947Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC92600.1934168507016165Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAACTC92290.19276934288609274Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA92080.1923307085594476No Hit
ATCTGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGA88060.18393399430652646Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCGGACTATGCCAGGCAAGAAATAGATCGGAAGAGCACACGTCTGAAC85640.17887925587566347Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGA78980.16496828151634635Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT74970.15659245461231305Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCTTGTCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGATCGGA70380.14700516147278367No Hit
ATAGTGGACAGTCCAAGAGTGATAAGATCGGAAGAGCACACGTCTGAACT63560.13275998953126072Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG63300.1322169184601763No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATCTCGTATG61790.12906292877810888TruSeq Adapter, Index 10 (97% over 38bp)
AAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCAC61580.12862429445146376No Hit
TCTGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAA60270.12588805174715362Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC60140.1256165162116114Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT60010.12534498067606917Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGATCGGAAGAG58290.12175235666735665No Hit
AGTTGAAACTGAGATGAAGAAAGAGTCCAGATCGGAAGAGCACACGTCTG55670.1162798712587364Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGATCGGAAGAGCA54610.11406581227662285No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC54430.11368983999664131Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC54110.11302144483222969Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA52910.11051496296568604No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC51360.1072774238880672No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT51120.10677612751475848Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATGGCCTGTTGAATCTGATTCGGACAGATCGGAAGAGCACACGTCTGA49380.10314172880827022Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCATGGGTCGTGATAGTGGACAGTCCAGATCGGAAGAGCACACGTCTGAA48900.10213913606165277Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA48340.10096944452393242No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)