FastQCFastQC Report
Fri 14 Jul 2023
SRR9691744_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691744_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4684367
Total Bases356 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTC997932.1303411965800287Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACT594041.2681329195598894Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCC570751.2184143556642764Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA466940.9968049044833593Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG381450.8143042592521039No Hit
ATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCC321250.6857916982166428Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA295400.6306081483367977No Hit
ATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACT278550.5946374398077692Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC262970.5613778766693558No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG243250.5192804065095669No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA242000.5166119563219534No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC238630.5094178146161478Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAA208900.4459513953539507Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC206320.4404437141667166No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG203690.43482929497197803No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA187700.4006944801720275No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC184960.394845237360779No Hit
AATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAAC178960.38203667646023465Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA178250.38052099675367024No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA176190.3761233908444834No Hit
ATTCGGACTATGCCAGGCAAGAAATCCAGATCGGAAGAGCACACGTCTGA175750.37518409637844347Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAACTCCA174590.37270777460433824Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTC174510.37253699379233096Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA169960.36282383510941824No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC162250.34636483435221876No Hit
AATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGA157370.3359472048197761Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC154500.3298204431890157No Hit
TGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTCCA147700.31530407416839884Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC140350.29961358706523206Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG120900.25809250214596763No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA120260.2567262556499096Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG117660.2511758792596737No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA105740.22572953827059236Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG102040.21783092571525672No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA98920.2111704740469737No Hit
TGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACT98600.21048735079894468Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAACTC92180.19678219063536226Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC90120.19238458472617537Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGA88760.189481310922052Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA88750.18945996332055112No Hit
ATTCGGACTATGCCAGGCAAGAAATAGATCGGAAGAGCACACGTCTGAAC83710.17870077216409389Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGA78200.16693824373709404Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT74530.15910367398626113Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCTTGTCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGATCGGA68940.147170364747254No Hit
ATAGTGGACAGTCCAAGAGTGATAAGATCGGAAGAGCACACGTCTGAACT62260.13291016694464802Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATCTCGTATG61950.1322483912981199TruSeq Adapter, Index 10 (97% over 38bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG61590.13147987764408725No Hit
AAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCAC61150.13054058317804731No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC59630.12729574774990945Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAA59410.12682610051688947Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT57590.12294083704372438Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGATCGGAAGAG57380.12249253741220532No Hit
AGTTGAAACTGAGATGAAGAAAGAGTCCAGATCGGAAGAGCACACGTCTG54580.11651520899195131Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGATCGGAAGAGCA53740.1147220104658751No Hit
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC52900.11292881193979891Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA51480.1098974525266701No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC50700.10823233960959933Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT50230.10722900233905669Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCATGGGTCGTGATAGTGGACAGTCCAGATCGGAAGAGCACACGTCTGAA48860.10430438093343242Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATGGCCTGTTGAATCTGATTCGGACAGATCGGAAGAGCACACGTCTGA48690.103941471707917Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC47600.10161458314431812No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)