FastQCFastQC Report
Fri 14 Jul 2023
SRR9691745_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691745_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4856302
Total Bases369 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTC1024902.110453592054201Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACT602421.2404912215097001Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCC577901.1900001276691605Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA469830.9674645440090011Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG395790.8150028560826736No Hit
ATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACTCC328240.6759052464200126Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA299700.617136248940037No Hit
ATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACT284590.586022038991809Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC270930.5578936400578053No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG255730.5265941039086943No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA243840.5021104535920542No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC240180.49457385475614984Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC213160.4389348108910855No Hit
ATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAA208610.42956554184645024Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG208440.42921548124478254No Hit
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA190970.393241606473403No Hit
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC190840.39297391307212776No Hit
AATCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAAC183160.3771594105967874Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA182300.3753885157883509No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA181940.37464721098481935No Hit
ATTCGGACTATGCCAGGCAAGAAATCCAGATCGGAAGAGCACACGTCTGA181750.3742559667829554Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTC181190.37310282597746186Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA175240.36085070491909277No Hit
ATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAACTCCA174840.3600270329151688Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC165970.34176210622815467No Hit
AATCTGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGA159390.3282127017636053Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC154070.31725786411141643No Hit
TGATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTCCA147970.30469686605157587Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC141990.2923829695929125Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG125760.25896247803369726No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG119700.24648384717424907No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA119220.24549544076954027Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA107720.22181487065672606Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCG105700.21765532703690998No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA103160.21242500981199275No Hit
TGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAACT97600.2009759689574495Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA93620.192780432518406No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC93590.19271865711811167Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGATTCGGACTATGCCAGGCAAGAGATCGGAAGAGCACACGTCTGAACTC91030.18744715629299824Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGA88190.18159908506513806Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCGGACTATGCCAGGCAAGAAATAGATCGGAAGAGCACACGTCTGAAC84490.17398011902884128Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAATCTGATTCGGACTATGCCAGGCAGATCGGAAGAGCACACGTCTGA78220.161069060367333Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCGGACTATGCCAGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT76430.1573831281497732Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCTTGTCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGATCGGA70600.1453781086925813No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG63880.13154041902665856No Hit
ATAGTGGACAGTCCAAGAGTGATAAGATCGGAAGAGCACACGTCTGAACT63860.13149923542646236Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC62610.12892526041419994Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCTAATCTCGTATG62530.12876052601341514TruSeq Adapter, Index 10 (97% over 38bp)
AAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCAC61940.1275456098076273No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT60620.12482749219467817Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGATTCGGACTATGCCAGGCAAGAAGATCGGAAGAGCACACGTCTGAA60050.1236537595890865Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGATCGGAAGAG57540.11848521776446357No Hit
TCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGATCGGAAGAGCA55290.11385206274239124No Hit
AGTTGAAACTGAGATGAAGAAAGAGTCCAGATCGGAAGAGCACACGTCTG54990.11323430873944826Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC54490.11220471873454327Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC52920.10897180611914169Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA51440.10592421970462297No Hit
TCATGGGTCGTGATAGTGGACAGTCCAGATCGGAAGAGCACACGTCTGAA51160.10534764930187619Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA50310.10359734629353777No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT50140.10324728569187007Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC49910.10277367428961379No Hit
AGATGGCCTGTTGAATCTGATTCGGACAGATCGGAAGAGCACACGTCTGA49650.10223828748706319Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)