FastQCFastQC Report
Fri 14 Jul 2023
SRR9691748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011773
Total Bases228.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG2728139.058219195138545No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC369991.228479038758897No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG312741.0383916716166857No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG234090.7772498126518831No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA210870.7001523687210158No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA127140.42214336870673846Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA122050.4052430246237017TruSeq Adapter, Index 4 (100% over 36bp)
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC119990.3984031997099383No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG100520.3337568933648054No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC74810.24839189407701046No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA71170.2363059898604576No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA70810.23511068065222712No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC62330.20695450819168643Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG61470.20409904730535802No Hit
ACGATGTAGATACTCTGGATATGGTCAGATCGGAAGAGCACACGTCTGAA58160.19310884319634977Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC55730.18504050604079392No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACATATC54540.18108934504692087TruSeq Adapter, Index 4 (97% over 37bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC53200.17664013854961846Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52150.17315382002561283No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG52000.17265577452218345No Hit
ATATCTTCAGGAGATCGTCATGGACAGATTCCCACGAGATCGGAAGAGCA51120.1697339075687311No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA50360.16721047701802227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA45930.15250153315007473No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA44730.14851716912263974No Hit
AGGGAGTATGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT44390.14738826598153315Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT44280.14702303261235158No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA43390.1440679626253373No Hit
ATGCGGCTATCACACAACAAATGAGATCGGAAGAGCACACGTCTGAACTC40690.13510314356360856Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA40440.13427306772455957Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA40080.13307775851632908No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC39320.13055432796562025Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC38740.12862855201902665Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC38420.127566054945044No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT37880.12577309113269824Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGTAGATCG35600.11820279948057175No Hit
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG33930.1126578928757247Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA33130.11000165019076802No Hit
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA33020.1096364168215865Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC32080.10651533166676241Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACATATCT31090.10322823134412853TruSeq Adapter, Index 4 (97% over 37bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG30570.10150167359890669TruSeq Adapter, Index 4 (100% over 35bp)
AAGACTGACTAAGCATGTAGTACCGAGATCGGAAGAGCACACGTCTGAAC30470.1011696432632871Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)