FastQCFastQC Report
Fri 14 Jul 2023
SRR9691749_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691749_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3079533
Total Bases234 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG2858789.283160790938107No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC388211.2606132163545578No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG332981.0812678415850716No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG246030.7989198362219208No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA219690.7133873869836758No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA132870.4314615235491875Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA127700.414673263770838TruSeq Adapter, Index 4 (100% over 36bp)
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC127100.41272491640778003No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG108130.3511246672790972No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC76230.2475375324765151No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA74700.24256924670071728No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA73770.23954930828797744No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC65250.21188277573255423Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG62440.20275801558223275No Hit
ACGATGTAGATACTCTGGATATGGTCAGATCGGAAGAGCACACGTCTGAA60620.1968480285809569Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC58700.19061331701917142No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACATATC57730.18746348878222768TruSeq Adapter, Index 4 (97% over 37bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC54580.17723466512617336Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG54020.17541620758731924No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA51790.1681748498879538Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATATCTTCAGGAGATCGTCATGGACAGATTCCCACGAGATCGGAAGAGCA51690.16785012532744414No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA48520.15755635675928786No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA47240.15339988238476418No Hit
AGGGAGTATGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT46360.15054230625227916Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT45180.14671055643826514No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA43830.14232677487138473Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA43680.14183968803062022No Hit
ATGCGGCTATCACACAACAAATGAGATCGGAAGAGCACACGTCTGAACTC43030.1397289783873074Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC42910.13933930891469584Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA42100.13670903997456757No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC41760.1356049764688347No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC40400.13118872244590332Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT38960.12651268877456417Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG37050.12031044966882966Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGTAGATCG36670.11907649633889293No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA35060.11384843091468738No Hit
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA33910.11011409846882628Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC33130.10758124689685092Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACATATCT33040.1072889947923922TruSeq Adapter, Index 4 (97% over 37bp)
TCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGT32840.1066395456713729Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG32550.10569784444589489TruSeq Adapter, Index 4 (100% over 35bp)
AAGACTGACTAAGCATGTAGTACCGAGATCGGAAGAGCACACGTCTGAAC32120.10430152883570333Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAAG31960.10378196953888788No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)