FastQCFastQC Report
Fri 14 Jul 2023
SRR9691750_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691750_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2896243
Total Bases220.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG2650029.149853793345379No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC358931.2392951834497312No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG306841.0594414902340723No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG224690.7757981633447194No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA202790.7001829611672777No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA122640.423445132193673Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC118100.4077696519249248No Hit
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA117520.4057670575293579TruSeq Adapter, Index 4 (100% over 36bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG98410.33978502494438484No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC70890.2447653736236911No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA67230.23212831243787208No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGAA66380.22919347582367916No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC61930.21382874296113963Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG58200.20094998934826946No Hit
ACGATGTAGATACTCTGGATATGGTCAGATCGGAAGAGCACACGTCTGAA56530.1951838985886198Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC54760.18907253293318274No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACATATC52500.1812693202883874TruSeq Adapter, Index 4 (97% over 37bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC49940.17243028295622984Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA49610.17129087580013141Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG49480.17084201843560778No Hit
ATATCTTCAGGAGATCGTCATGGACAGATTCCCACGAGATCGGAAGAGCA47220.16303880579081245No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA44530.15375091109413125No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA44510.15368185611497376No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT43270.14940044740720998No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA41940.14480829129323747No Hit
AGGGAGTATGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT41040.14170081723115083Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40590.14014708020010752No Hit
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA39900.13776468341917442Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCGGCTATCACACAACAAATGAGATCGGAAGAGCACACGTCTGAACTC39770.13731582605465079Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAAGATCGGA39250.1355203965965563No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC39000.1346572093570878Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC37900.13085918550342635Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC37820.1305829655867964No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT36990.12771718395176096Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGACTGACTAAGCATGTAGTACCGAGGAGATCGGAAGAGCACACGTCTG34060.11760062950519001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGTAGATCG33600.11601236498456793No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGAGATCGGAAGA31820.10986647183955213No Hit
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACATATCT31490.10872706468345371TruSeq Adapter, Index 4 (97% over 37bp)
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA30550.10548148066305209Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCAGATCGGAAG30310.10465282091316232No Hit
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC30220.10434207350695367Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGACTGACTAAGCATGTAGTACCGAGATCGGAAGAGCACACGTCTGAAC29830.10299550141338279Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG29030.10023330224708356TruSeq Adapter, Index 4 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)