FastQCFastQC Report
Fri 14 Jul 2023
SRR9691751_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691751_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2896104
Total Bases220.1 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG1302024.495763964277526No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC355751.2283743953946407No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG166520.5749793515702475No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA124040.428299536204501No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA120460.415938101670382Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA91920.31739191686486395No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG83720.28907801653531784No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC78970.272676671832227Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGA76740.26497667210846015TruSeq Adapter, Index 2 (97% over 36bp)
ACGATGTAGATACTCTGGATATGGTCAGATCGGAAGAGCACACGTCTGAA73150.2525807084275979Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG69450.23980492413255877No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA67830.23421120236013626No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC60450.20872869206354466No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA57230.1976103068121863Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC56790.19609102435547893No Hit
TTCTTGGACTGGACCATAAAATTTCAGATCGGAAGAGCACACGTCTGAAC54470.18808026231102198Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA53580.1850071682508639Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG52110.1799313836795916No Hit
ATATCTTCAGGAGATCGTCATGGACAGATTCCCACGAGATCGGAAGAGCA51880.1791372133044946No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA50320.17375066641253215No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT46570.16080223638377628No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC45900.15848878355197188No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG45540.15724573426921132No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC45170.1559681558397074No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC44660.15420716935579662Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA43210.149200443078011No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACGGATC42000.14502241632206578TruSeq Adapter, Index 4 (97% over 37bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA38570.13317891898909706No Hit
AGGGAGTATGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG37360.12900089223315184Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA36680.1266529102546041Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC36140.12478833633046327Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGT35520.12264752923237562Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG34850.12033407640057124No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC33200.11463676718791868Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG33120.11436053401397187TruSeq Adapter, Index 11 (100% over 35bp)
ATGCGGCTATCACACAACAAATGAGATCGGAAGAGCACACGTCTGAACTC30240.10441613975188735Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT30030.10369102767027703Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG29940.10338026534958689TruSeq Adapter, Index 11 (100% over 35bp)
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC29790.10286232814843667Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACGATCCGGTACGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA29200.10082510849057907Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)