FastQCFastQC Report
Fri 14 Jul 2023
SRR9691752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2854881
Total Bases216.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG1246864.367467505650848No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC347021.2155322761263954No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG160290.5614594793968646No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA118800.4161294288623589No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA113970.39921103541618724Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA89830.31465409591503113No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG79540.278610562051448No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC75660.2650198029269872Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGA74480.2608865308221253TruSeq Adapter, Index 2 (97% over 36bp)
ACGATGTAGATACTCTGGATATGGTCAGATCGGAAGAGCACACGTCTGAA71330.24985279596592644Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG66340.23237395884451928No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA66030.23128809922375046No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC59450.2082398530796905No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA56420.19762645097991827Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC54760.19181184784934993No Hit
TTCTTGGACTGGACCATAAAATTTCAGATCGGAAGAGCACACGTCTGAAC53100.18599724471878162Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52030.18224927764064422No Hit
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA51380.17997247521Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG50520.17696009045560918No Hit
ATATCTTCAGGAGATCGTCATGGACAGATTCCCACGAGATCGGAAGAGCA49600.17373753932300506No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA47880.16771276981422345No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT44750.15674909041742896No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG44570.15611859128278902No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC44520.15594345263427792No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC42580.1491480730720475No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC42480.1487977957750253Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA41020.14368374723850136No Hit
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACGGATC39020.1366782012980576TruSeq Adapter, Index 4 (97% over 37bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA37320.1307234872486804No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC35500.1243484404428766Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA35400.1239981631458544Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGAGTATGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG34250.11996997423009927Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG33190.11625703488166407No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC33130.11604686850345076Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGT32730.114645759315362Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG31410.11002209899466914TruSeq Adapter, Index 11 (100% over 35bp)
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC29560.10354196899975865Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG29450.10315666397303425TruSeq Adapter, Index 11 (100% over 35bp)
ATGCGGCTATCACACAACAAATGAGATCGGAAGAGCACACGTCTGAACTC29060.10179058251464772Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)