FastQCFastQC Report
Fri 14 Jul 2023
SRR9691754_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691754_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926708
Total Bases222.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG1316194.497168832695301No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC361661.2357228667841138No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCAGATCG168450.5755613474251617No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA124670.42597348283463876Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA122770.4194815471854384No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA97190.3320795924977825No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG83060.28380009211715007No Hit
TGCGTCTGGATCGTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC79940.2731396504195157Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGAGTATGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGA78900.26958616985363765TruSeq Adapter, Index 2 (97% over 36bp)
ACGATGTAGATACTCTGGATATGGTCAGATCGGAAGAGCACACGTCTGAA75700.2586523834970896Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA67750.23148875801754054No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG66990.22889198375786035No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC63190.21590811245945957No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA58500.19988328183064386Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCTGAGGCGTGATGACGAGGCACAGCGAAGCTCTTGATCGAAGCCCC57340.19591978427639517No Hit
TTCTTGGACTGGACCATAAAATTTCAGATCGGAAGAGCACACGTCTGAAC54670.18679690628515042Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCA54340.18566935956713138Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATATCTTCAGGAGATCGTCATGGACAGATTCCCACGAGATCGGAAGAGCA53400.18245755982489542No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAAGATCGG52810.18044164296540688No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTAGATCGGAA51350.1754531029402318No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT48430.16547602288988172No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAGATCGGAAG47200.16127334875908358No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC46140.15765153202847704No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC45660.15601146407499483No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC45270.15467890886279054Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACGGATC43030.1470252584132069TruSeq Adapter, Index 4 (97% over 37bp)
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA42570.14545352662445316No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTAGATCGGA39780.1359206316448378No Hit
AGGGAGTATGCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG37410.1278227961245194Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA36820.1258068792650309Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTGTTCGGTCTCTTTTTAGATCGGAAGAGCACACGTCTGAACTCCAGT36480.12464516446464766Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG35850.12249257527570225No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC35440.12109168389876954Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC34890.11921243936873784Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG32190.10998705713040043TruSeq Adapter, Index 11 (100% over 35bp)
ATGCGGCTATCACACAACAAATGAGATCGGAAGAGCACACGTCTGAACTC31210.1066385850587076Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC30910.10561354258778122Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG30310.10356345764592848TruSeq Adapter, Index 11 (100% over 35bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT30040.10264091942209473Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)