FastQCFastQC Report
Fri 14 Jul 2023
SRR9691755_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691755_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7575068
Total Bases575.7 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT2342693.0926323037628176No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG1059101.398139264228387No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA946751.2498237639582905Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC826521.0911057168067666No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC809611.0687824848569016No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG705180.9309223362747371No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA572270.755465165461221No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA537760.7099078186492848No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA510860.6743965862748691No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA452760.5976976048267818No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC452400.5972223615682394No Hit
TCTGCGCACCGGTGTTGATGATGTTAAGATCGGAAGAGCACACGTCTGAA433900.5728001385598123Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA416790.5502128825774237No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACAGATCGGAAGAGCACACGT393410.5193484731754223No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG383330.5060416619362361No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC353840.4671113183406407No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACTGAGATCGGAAGAGCACAC293390.38731005451040174No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACTATCTCGTATG292060.3855542946941203TruSeq Adapter, Index 19 (97% over 37bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA291720.3851054538388302No Hit
AAGCACTGGTTTGTAGCGGTTATGAAGTTCGTGAGATCGGAAGAGCACAC283320.3740164444728417No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC275950.36428715887434937No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC272040.3591254890385142Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTGTCTGGAAGCAGTTAGTCCGGATCTGCCGAGCTAGATCGGAAGAGC232220.30655830416307817No Hit
ATGCACTGGTTAGCGCATTTTGTCTGGAAGCAGATCGGAAGAGCACACGT231650.30580583567038605No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG229220.3025979436752251No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG225180.29726465821824966Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGTGGCTACAGCTCAGTTGGTAGAGCCCTGGAAGATCGGAAGAGCACACG210070.2773176425610965No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA199200.2629679363934423No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG193790.2558260863136806No Hit
AGCACTGGTTTGTAGCGGTTATGAAGTTCGTGAGATCGGAAGAGCACACG187410.2474037196761798No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACAGATCGGAAGAGCACACG181240.2392585782728287No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT176740.23331803754104916Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT174830.23079660803044938No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT172130.22723228359138162Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC155470.20523908168217103No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT155260.20496185644802134No Hit
GGTGGCTACAGCTCAGTTGGTAGAGCCCTGGATTGTAGATCGGAAGAGCA153470.20259884135693565No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC139670.18438118311281165No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG139550.18422276869329754No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA138480.18281024011929664No Hit
AGCTTTCGTCAGGCACTGTATCATATTACCACCCTGCTGCGAGATCGGAA136650.18039442022170626No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA128590.16975425171100775Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGGATCTGCCGAGCTTTCGTCAGGAGATCGGAAGAGCACACGTCTG127440.1682361135239974Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCT123320.16279721845401257Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTCGTGATCTGCGTACCTATATTATGCCTGAGATCGGAAGAGCACACG122890.16222956678408695No Hit
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA121820.16081703821008603Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA119320.15751673780354183Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT119300.15749033540028948Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACTGTATCAGATCGGAAGAGC114960.15176101389452873No Hit
GGTGGCTACAGCTCAGTTGGTAGAGCCCTGGATTGTGAGATCGGAAGAGC114040.15054650334492048No Hit
GGTGGCTACAGCTCAGTTGGTAGAGCCCTGGATAGATCGGAAGAGCACAC110490.1458600767676277No Hit
TTCGTGATCTGCGTACCTATATTATGCCTGAGATCGGAAGAGCACACGTC109070.1439855061367106No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC108280.14294261120824261No Hit
ATCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGA107960.14252017275620496Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGTCAGGCACTGTATCATATTACCACCCAGATCGGAAGAGCACACGT107210.1415300826342417No Hit
TTTCGTCAGGCACTGTATCATATTACCACCAGATCGGAAGAGCACACGTC103030.1360119803544998No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT99180.1309295177284217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT95430.1259790671186054No Hit
TCTGCGCACCGGTGTTGATGATGTTAGATCGGAAGAGCACACGTCTGAAC92230.12175468259822882Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTAAGATCGGAAGAGCACACG91120.12028934921772319No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC89500.11815075455428256No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGGCAGATCGGAAGAGCACACGTCT89120.11764910889248784Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGAGATCGG86600.11432240608269127No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGAGATCGGAAGAGCA85980.1135039315818683No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG85350.11267225587941916No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA85070.11230262223388622Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGAGATCGGAAGAGC82250.10857988337530436No Hit
AATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCCCGAGATCGGAAGAGC82220.10854027977042582No Hit
ACTGGTTTGTAGCGGTTATGAAGTTCGTGAGATCGGAAGAGCACACGTCT79420.10484394331509632Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA78560.1037086399752451Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACTAGATCGGAAGAGCACACG78230.10327300032158128No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGACTAGATCGGAAGAGCACAC76770.10134562488415945No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)