FastQCFastQC Report
Fri 14 Jul 2023
SRR9691756_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691756_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7263241
Total Bases552 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT2276443.1341931239786756No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG1024801.4109403777184317No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA932551.2839309613986374Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC805641.1092018012344627No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC773641.0651443343267832No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG686680.9454181680051645No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA545970.7516892252370533No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA525450.7234373745825039No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA492810.678498758336671No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA439540.6051568438937934No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC432390.5953127536316087No Hit
TCTGCGCACCGGTGTTGATGATGTTAAGATCGGAAGAGCACACGTCTGAA430000.5920222115719415Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA405100.5577399951344035No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACAGATCGGAAGAGCACACGT382680.5268722323822106No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG375700.5172621974129731No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC345960.4763162891056486No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACTATCTCGTATG286310.3941904171980525TruSeq Adapter, Index 19 (97% over 37bp)
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACTGAGATCGGAAGAGCACAC285850.3935570911112546No Hit
AAGCACTGGTTTGTAGCGGTTATGAAGTTCGTGAGATCGGAAGAGCACAC271850.3742819493391449No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA271040.37316674470804423No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC268600.3698073628563337No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC267820.36873346210045904Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG227570.31331742950564356No Hit
TTTTGTCTGGAAGCAGTTAGTCCGGATCTGCCGAGCTAGATCGGAAGAGC226200.3114312192036585No Hit
ATGCACTGGTTAGCGCATTTTGTCTGGAAGCAGATCGGAAGAGCACACGT224880.3096138486937168No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG218270.3005132281855992Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGTGGCTACAGCTCAGTTGGTAGAGCCCTGGAAGATCGGAAGAGCACACG206040.2836750150518205No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA193590.26653390683305156No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG188180.259085441333972No Hit
AGCACTGGTTTGTAGCGGTTATGAAGTTCGTGAGATCGGAAGAGCACACG181010.24921381515497007No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACAGATCGGAAGAGCACACG174500.24025087423093905No Hit
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT168280.23168720410075885No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT167840.23108141393077827Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT162870.22423873860167934Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT151810.20901137660171268No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC149150.2053490996650118No Hit
GGTGGCTACAGCTCAGTTGGTAGAGCCCTGGATTGTAGATCGGAAGAGCA148260.20412375136664196No Hit
AGCTTTCGTCAGGCACTGTATCATATTACCACCCTGCTGCGAGATCGGAA134460.18512396876270526No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA134000.18449064267590734No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG133740.18413267575728248No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC133150.18332036621117212No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGGAGATCGGAAGAGCACACGTCTG123330.169800231053878Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA122870.16916690496708012Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTCGTGATCTGCGTACCTATATTATGCCTGAGATCGGAAGAGCACACG120320.16565607557287443No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCT118700.16342566631067315Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA118140.16265466063978876Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT117360.16158075988391407Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTGGCTACAGCTCAGTTGGTAGAGCCCTGGATTGTGAGATCGGAAGAGC113550.1563351677302185No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACTGTATCAGATCGGAAGAGC113430.1561699522293147No Hit
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA113310.15600473672841092Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGTGATCTGCGTACCTATATTATGCCTGAGATCGGAAGAGCACACGTC107690.14826714410274972No Hit
GGTGGCTACAGCTCAGTTGGTAGAGCCCTGGATAGATCGGAAGAGCACAC106950.14724831518050963No Hit
TTTCGTCAGGCACTGTATCATATTACCACCCAGATCGGAAGAGCACACGT105710.14554108833783705No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC104580.14398530903765966No Hit
ATCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGA103620.14266358503042925Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGTCAGGCACTGTATCATATTACCACCAGATCGGAAGAGCACACGTC98800.13602742907746004No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT94560.13018981471219251Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT92650.12756013465614044No Hit
TCTGCGCACCGGTGTTGATGATGTTAGATCGGAAGAGCACACGTCTGAAC88640.12203918333427186Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTAAGATCGGAAGAGCACACG87330.12023558078273873No Hit
AAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGAGATCGG86060.1184870500648402No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGGCAGATCGGAAGAGCACACGTCT85670.11795009968690286Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA85350.11750952501782606Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC84820.11677982322216762No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG83770.1153341875892594No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGAGATCGGAAGAGCA81640.11240161244821699No Hit
AATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCCCGAGATCGGAAGAGC80560.11091467294008281No Hit
CACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGAGATCGGAAGAGC78900.10862919184424695No Hit
ACTGGTTTGTAGCGGTTATGAAGTTCGTGAGATCGGAAGAGCACACGTCT78470.10803716963267501Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACTAGATCGGAAGAGCACACG76410.10520097020049314No Hit
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA75630.10412706944461846Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGACTAGATCGGAAGAGCACAC75110.10341113560736867No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT72990.10049232842473489TruSeq Adapter, Index 3 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)