FastQCFastQC Report
Fri 14 Jul 2023
SRR9691757_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691757_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7587704
Total Bases576.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT2355433.104272385954961No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG1075511.4174380023258684No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA965801.272848809073206Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC838541.1051300894183536No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC819511.0800500388523326No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG712720.9393091770580403No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA563850.7431101687677854No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA555640.7322900313454505No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA509590.6715997355721837No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA461230.607865040597261No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC450680.5939609663213009No Hit
TCTGCGCACCGGTGTTGATGATGTTAAGATCGGAAGAGCACACGTCTGAA445950.5877271965274344Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA424150.5589965027628911No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACAGATCGGAAGAGCACACGT397990.5245196702454392No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG388370.5118412631805352No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC353470.4658457947226196No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACTATCTCGTATG296550.3908296897190507TruSeq Adapter, Index 19 (97% over 37bp)
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACTGAGATCGGAAGAGCACAC293330.38658598174098513No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA287810.37931105377858704No Hit
AAGCACTGGTTTGTAGCGGTTATGAAGTTCGTGAGATCGGAAGAGCACAC283590.373749424068203No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC280720.3699669886964489No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC280320.3694398200035215Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGCACTGGTTAGCGCATTTTGTCTGGAAGCAGATCGGAAGAGCACACGT236360.31150398065080026No Hit
TTTTGTCTGGAAGCAGTTAGTCCGGATCTGCCGAGCTAGATCGGAAGAGC235460.31031785109171367No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG234920.30960617335626167No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG229110.30194904809149115Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGTGGCTACAGCTCAGTTGGTAGAGCCCTGGAAGATCGGAAGAGCACACG217400.28651618460604156No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA204360.26933048521660835No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAAGATCGGAAG194750.25666525736902757No Hit
AGCACTGGTTTGTAGCGGTTATGAAGTTCGTGAGATCGGAAGAGCACACG192570.25379218799257325No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACAGATCGGAAGAGCACACG182140.2400462643244913No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT177140.23345665566289883Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT176990.23325896740305105No Hit
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT171970.2266430003068122Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTGGCTACAGCTCAGTTGGTAGAGCCCTGGATTGTAGATCGGAAGAGCA161830.2132792739411026No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC158480.20886423613783564No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT156670.20647879780233916No Hit
GCGGGCATCGTATAATGGCTATTACCTCAGCCTTAGATCGGAAGAGCACA142890.18831783633099025No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG141400.1863541329498357No Hit
AGCTTTCGTCAGGCACTGTATCATATTACCACCCTGCTGCGAGATCGGAA139960.18445632565529704No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC139010.18320430000959448No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA128620.16951109321080526Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTCGTGATCTGCGTACCTATATTATGCCTGAGATCGGAAGAGCACACG126160.16626900574930176No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGGAGATCGGAAGAGCACACGTCTG125520.16542553584061792Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCT124460.1640285388043603Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATGTGGCGCTCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCA124350.16388356741380528Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA121610.1602724618672526Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGGCTACAGCTCAGTTGGTAGAGCCCTGGATTGTGAGATCGGAAGAGC121100.15960032178377015No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT120620.1589677193522573Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACTGTATCAGATCGGAAGAGC117270.15455268154899032No Hit
TTCGTGATCTGCGTACCTATATTATGCCTGAGATCGGAAGAGCACACGTC114700.1511656226969318No Hit
GGTGGCTACAGCTCAGTTGGTAGAGCCCTGGATAGATCGGAAGAGCACAC112850.14872746749214255No Hit
TTTCGTCAGGCACTGTATCATATTACCACCCAGATCGGAAGAGCACACGT109170.14387751551721048No Hit
ATCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGA109070.14374572334397862Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC106950.1409517292714634No Hit
TTTCGTCAGGCACTGTATCATATTACCACCAGATCGGAAGAGCACACGTC103240.13606223964456177No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT97380.12833921829317538Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT97180.12807563394671168No Hit
TCTGCGCACCGGTGTTGATGATGTTAGATCGGAAGAGCACACGTCTGAAC93550.12329157805839554Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTAAGATCGGAAGAGCACACG92320.12167053432764378No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGGCAGATCGGAAGAGCACACGTCT90420.11916648303623863Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC88800.11703144982988267No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGAGATCGGAAGAGCA88630.11680740313538852No Hit
AAACCAGGCGTTGAACTGGTTGAACCGACCAGCGGTAATACCGAGATCGG87550.11538404766448455No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA87090.11477780366761803Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG86440.113921154541611No Hit
AATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCCCGAGATCGGAAGAGC82860.10920299473991077No Hit
ACTGGTTTGTAGCGGTTATGAAGTTCGTGAGATCGGAAGAGCACACGTCT82600.10886033508950797Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGGGTACCGAGAACCTGTACTTCCAGATCGGAAGAGCACACGTCTGAA80610.10623767084219417Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGAGATCGGAAGAGC79950.10536784249886395No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACTAGATCGGAAGAGCACACG79640.10495928676184521No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGACTAGATCGGAAGAGCACAC77860.10261338607831828No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)