FastQCFastQC Report
Fri 14 Jul 2023
SRR9691759_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691759_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6407408
Total Bases486.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG2056043.208848258141202Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1971463.076844802141521Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGAATTTCGGACGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCA1944813.0352523204390915Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCGGACGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC757791.1826779253014637Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGGCTGAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGA636510.9933970179517209TruSeq Adapter, Index 2 (97% over 36bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA563210.878998184601324Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC543210.847784314655786No Hit
GGAAGATCGTCGTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA510000.79595368361122Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA474270.7401901049535163No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG435090.6790421337302073No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACAGATCGGAAGAGCACACGT422540.6594554303393821No Hit
AGGGTGAGGTCGGTGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT407670.6362479180348746Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCGGACGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG403340.6294901151916656Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACCGGGTGTTGGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT397100.6197513877686578Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACTGAGATCGGAAGAGCACAC367000.572774513500623No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA360840.5631606415573973No Hit
ATTTCGGACGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA353440.5516115096775482Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAACGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG324980.5071941727450475TruSeq Adapter, Index 11 (100% over 35bp)
AGTCCGGATCTGCCGAGCTTTCGTCAGGAGATCGGAAGAGCACACGTCTG276180.4310323300779348Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA270420.4220427355336199No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG266430.41581556847948503No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG264710.41313117566416874No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC263120.4106496730034984No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAACTATCTCGTATG258300.4031271303466238TruSeq Adapter, Index 2 (97% over 36bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC248710.3881600797077383Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGCTGAGGCGTGATGACGAGGCAGATCGGAAGAGCACACGTCTGAACT239540.3738485203377091Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC228110.3560097936638341Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT217310.3391543038932436Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG200090.3122791618701353Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG192450.3003554635509398No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC188750.29458089761101525Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGACGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG186310.2907728054776596TruSeq Adapter, Index 11 (100% over 35bp)
TAGGAATTTCGGACGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCC184030.28721442430386823Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGTCGGGGTCGCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT177470.2769762749617318Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATAGATCGGAA167940.26210286593268295No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC167320.2611352359643712No Hit
AGGAATTTCGGACGCGGGTTCAACTCCCGCCAGATCGGAAGAGCACACGT166950.26055777937037883No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG157160.24527859003203792Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGCGCACCGGTGTTGATGATGTTAGATCGGAAGAGCACACGTCTGAAC156390.24407685603913468Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA155280.24234448625715735Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT153330.23930113393746738No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACTAGATCGGAAGAGCACACG146810.229125412335222No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC146750.22903177072538536No Hit
AGGAGGTCTGCGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC143390.22378784057453496Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGCTGTGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG139770.2181381301143926Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC134670.21017859327828037No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC131920.2058866861607689No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA128370.2003462242454359No Hit
AGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAGATCGGAAGAGC124520.19433755428091984No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG123710.19307339254812556TruSeq Adapter, Index 11 (100% over 35bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACAGATCGGAAGAGCACACG120340.1878138554623024No Hit
AACCGGAGTAGGGGCAACTCTAGATCGGAAGAGCACACGTCTGAACTCCA113810.1776225269250842Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAACGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAC107600.16793062030699465TruSeq Adapter, Index 2 (97% over 36bp)
ATGCACTGGTTAGCGCATTTTGTCTGGAAGCAGATCGGAAGAGCACACGT103960.16224969597690675No Hit
ACCGGGTGTTGGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC100380.15666241325665542Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA95350.1488121249653526No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA91330.14253813710629948Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA90230.14082137425929486Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAACGGCGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAA88070.13745027630517676TruSeq Adapter, Index 2 (97% over 36bp)
ACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCTGTCGGGGGTCGGAGG85510.13345490095214788No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC83760.1307236873319133No Hit
AAGCACTGGTTTGTAGCGGTTATGAAGTTCGTGAGATCGGAAGAGCACAC82880.12935027705430963No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACAGATCGGAAGAGCACACG82160.12822657773627028No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGATCGGAAGAGCACACGTCTGAAC80300.1253236878313352Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGGATCTGCCGAGCTTTCGTCAAGATCGGAAGAGCACACGTCTGAA79400.12391906368378602Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT77480.12092253216901437No Hit
CCGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC75180.1173329371252775Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACTGTATCAGATCGGAAGAGC73650.11494507607444383No Hit
TTTCGGACGCGGGTTCAACTCCCGCCAGATCGGAAGAGCACACGTCTGAA72780.11358727273181293Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC72290.11282253291814726No Hit
TCTGCGCACCGGTGTTGATGATGTTAAGATCGGAAGAGCACACGTCTGAA71210.11113698394108817Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTCTGAACTCC70790.11048149267223188Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTCAGTCGGTAGAACGGCGGAAGATCGGAAGAGCACACGTCTGAACTC70290.10970114592359344Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCT68350.10667340053887625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA67520.10537802493613643Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGAGATCGGAAGAGCA67360.10512831397657212No Hit
ACCCGAAGGTCGTCGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT66510.10380172450388675Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAAGGAGATCAACAATGAAAGCAGATCGGAAGAGCACACGTCTGAACT65570.10233467261644645Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)