FastQCFastQC Report
Fri 14 Jul 2023
SRR9691760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6727224
Total Bases511.2 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG2131463.1684094360467254Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGACGTGGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2079653.091394013340421Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGAATTTCGGACGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCA2056743.0573383612616434Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCGGACGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC794651.1812450425316594Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGGCTGAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGA670640.9969045181192123TruSeq Adapter, Index 2 (97% over 36bp)
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA597510.8881969739672708Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC581960.8650819416746046No Hit
GGAAGATCGTCGTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA530410.788453008254222Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA510840.759362257002294No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG463690.689273911497521No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACAGATCGGAAGAGCACACGT445600.6623831761808436No Hit
AGGGTGAGGTCGGTGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT427850.6359978499303724Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCGGACGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG417520.620642333301225Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACCGGGTGTTGGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT416580.6192450258828901Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACTGAGATCGGAAGAGCACAC389890.5795704141857028No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA379440.5640365178861295No Hit
ATTTCGGACGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA370510.5507620974119488Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAACGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG337380.5015144434019143TruSeq Adapter, Index 11 (100% over 35bp)
AGTCCGGATCTGCCGAGCTTTCGTCAGGAGATCGGAAGAGCACACGTCTG293780.43670316314723573Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA288180.4283787785273688No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC282580.4200543939075018No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG281760.4188354661595927No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG280650.4171854542081548No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAACTATCTCGTATG271400.4034353546128388TruSeq Adapter, Index 2 (97% over 36bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC259140.38521089828434435Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGCTGAGGCGTGATGACGAGGCAGATCGGAAGAGCACACGTCTGAACT249700.37117836421085426Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGCTGAGGCGTGATGACGAGGAGATCGGAAGAGCACACGTCTGAACTC242320.3602080144796725Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT228150.3391443483969019Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG206190.30650086870899496Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGGCATCGTATAATGGCTATTACCTCAGCCAGATCGGAAGAGCACACG202730.30135758821172004No Hit
GGGGCTGATTCTGGATTCGACGGAGATCGGAAGAGCACACGTCTGAACTC195360.29040210345307366Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGACGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG193820.2881128976826102TruSeq Adapter, Index 11 (100% over 35bp)
TAGGAATTTCGGACGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCC190210.28274664259730314Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGTCGGGGTCGCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT186370.27703849314368006Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGATAGATCGGAA179520.2668559869568785No Hit
AGGAATTTCGGACGCGGGTTCAACTCCCGCCAGATCGGAAGAGCACACGT173960.25859106222715345No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC171570.25503833379117447No Hit
ACGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG168260.2501180278819317Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTGCGCACCGGTGTTGATGATGTTAGATCGGAAGAGCACACGTCTGAAC165060.2453612366705791Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT159790.2375273961443829No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA159770.23749766619931192Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC155090.23054085905270882No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACTAGATCGGAAGAGCACACG153630.2283705730625292No Hit
AGGAGGTCTGCGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC150840.22422324572513122Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGCTGTGCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG143360.21310424626859462Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTC141730.21068125574531188No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC140620.20903124379387394No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA138160.2053744605501467No Hit
CGGACGTGGGGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG133920.19907171219510456TruSeq Adapter, Index 11 (100% over 35bp)
AGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAGATCGGAAGAGC131000.19473114021474533No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACAGATCGGAAGAGCACACG124960.18575269680331738No Hit
AACCGGAGTAGGGGCAACTCTAGATCGGAAGAGCACACGTCTGAACTCCA124840.1855743171328917Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAACGGCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAC113320.16844986877202245TruSeq Adapter, Index 2 (97% over 36bp)
ATGCACTGGTTAGCGCATTTTGTCTGGAAGCAGATCGGAAGAGCACACGT109800.16321739843953464No Hit
ACCGGGTGTTGGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC106190.15785114335422754Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA98600.14656862919980068No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGAGATCGGAAGAGCACACGTCTGA93840.13949290227291378Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCTGAGGCGTGATGACGAGGCACAGATCGGAAGAGCACACGTCTGAA92080.13687666710666985Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCTGTCGGGGGTCGGAGG91130.13546449471579955No Hit
AGAACGGCGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAA90790.13495908564959336TruSeq Adapter, Index 2 (97% over 36bp)
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC89470.13299690927491042No Hit
AAGCACTGGTTTGTAGCGGTTATGAAGTTCGTGAGATCGGAAGAGCACAC88390.13139149224107893No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACAGATCGGAAGAGCACACG85120.12653064622197804No Hit
AGTCCGGATCTGCCGAGCTTTCGTCAGATCGGAAGAGCACACGTCTGAAC83030.12342386696206341Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGGATCTGCCGAGCTTTCGTCAAGATCGGAAGAGCACACGTCTGAA82980.12334954209938602Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGGATCTGCCGAGCTTTCGTCAGGCACTGTATCAGATCGGAAGAGC79440.11808734182182726No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT79320.11790896215140154No Hit
TCTGCGCACCGGTGTTGATGATGTTAAGATCGGAAGAGCACACGTCTGAA77000.11446028852317093Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGGACGCGGGTTCAACTCCCGCCAGATCGGAAGAGCACACGTCTGAA75680.11249811214848801Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAGATCGGAAGAGCACACGTC75070.11159134882382392No Hit
AGTTCAGTCGGTAGAACGGCGGAGATCGGAAGAGCACACGTCTGAACTCC73630.10945079277871526Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGAGATCGGAAGAGCACACGTCT72300.10747375143149686Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTGACTCTGTGACTCTGGTGCACAGATCGGAAGAGCACACGTCTGAA72300.10747375143149686Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTCAGTCGGTAGAACGGCGGAAGATCGGAAGAGCACACGTCTGAACTC71530.10632914854626514Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGAGATCGGAAGAGCA70900.10539265527653012No Hit
AAAAAGGAGATCAACAATGAAAGCAGATCGGAAGAGCACACGTCTGAACT69740.10366831846241481Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAACTAT67890.10091829854335162TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph