FastQCFastQC Report
Fri 14 Jul 2023
SRR9691764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4150257
Total Bases315.4 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA679571.6374166708230358No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC636861.5345073811091698No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG373790.9006430204201813No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA300140.7231841305249289Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAGATCGGAAGAGCACACG280270.6753075773379817No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA279440.6733077011857338No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA269750.6499597494805743No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC240440.5793376169234821No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG210740.5077757835237673No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA198610.478548677828867No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC190310.45854991630638775No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG178200.4293710003982886No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA149870.36111016739445295Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAACGTGATGGTAATGCAGTTCTGAATCTGCTGTTAGATCGGAAGAGC149420.3600258971914269No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA144260.347592932196729Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT122740.29574072159868653No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG104020.2506350811528057No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG85550.20613181304193934No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA80700.1944457897426593No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA77900.18769921959049765No Hit
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGAGATCGGAAGAGCA77570.1869040881082786No Hit
AGCAGCGTTCGTCGTGTTAGTGATGAGATCGGAAGAGCACACGTCTGAAC74880.18042256178352328Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA74700.1799888537023129Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC72210.17398922524556915Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA71720.17280857546894085No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC67710.1631465232153093Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGGTCTGGCATCACTGGGTGCAAGTGATGAAGAAATCGAAAAACTGAG65570.15799021602758576No Hit
ATTCGATCCGTATACCCTGGCAATTGAGATCGGAAGAGCACACGTCTGAA62310.15013528077899754Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC60400.14553315613948728No Hit
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC59240.14273814850502028No Hit
ATTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAGATCGGAAGAGCACAC58950.14203939659640355No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA57080.13753365153049557No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC56850.1369794689822823No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC54970.13244962902297375No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT54710.13182316179455875Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG54150.1304738477641264No Hit
AGGTTTTCGTCGTGCAGTTAGCGAACAGGATGAGATCGGAAGAGCACACG53020.12775112480986117No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAGATCGGAAGAGCAC52700.1269800882210427No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG50280.12114912401810296No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG50250.12107683933790124No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT49080.11825773681003368Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA48430.11669156873899617No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG48360.11652290448519213No Hit
AGGAACGTGATGGTAATGCAGTTCTGAATCTGCTGAGATCGGAAGAGCAC47380.11416160493193554No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGATCGGAAGAGCA47320.11401703557153207No Hit
AGGACTGGATGGATTCCAGTAACCCCGGAACCTGGACGGATGAGATCGGA46890.11298095515530726No Hit
ATGCGCGCGGCGATACCGAAGAACTTACCGAGATCGGAAGAGCACACGTC45620.10992090369343394No Hit
ACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAGATCGGAAGAGCA44650.10758369903357792No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA44630.10753550924677677No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA43120.10389718034328958No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT41670.10040342080020587No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)