FastQCFastQC Report
Fri 14 Jul 2023
SRR9691765_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691765_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4008595
Total Bases304.6 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA664161.6568398653393521No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC611461.5253723561497232No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG356720.889887853474846No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA295080.7361182658761986Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAGATCGGAAGAGCACACG279950.6983743680765954No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAGA269230.6716318311029176No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA262100.6538450504478501No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCAC230430.5748398129519195No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG203350.5072849714176663No Hit
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA194290.48468353625147964No Hit
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC186700.4657492213606014No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG175480.4377593645653901No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA146920.3665124563593978Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAACGTGATGGTAATGCAGTTCTGAATCTGCTGTTAGATCGGAAGAGC143900.35897864463733553No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA142330.3550620603977204Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT122130.3046703396077678No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCTAGATCGGAAGAGCACACG101080.2521581751212083No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG81030.20214065027771574No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA75430.1881706682765408No Hit
ATCGTGACTCTGTGACTCTGGTGCACAAGATCGGAAGAGCACACGTCTGA75230.18767174034792738Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCAGCGTTCGTCGTGTTAGTGATGAGATCGGAAGAGCACACGTCTGAAC73970.184528494397663Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGAGATCGGAAGAGCA72700.18136030205096798No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA70880.1768200579005861No Hit
ATCAGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACA70410.17564757726834465No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC69940.17447509663610317Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC67790.16911162140350922Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGGTCTGGCATCACTGGGTGCAAGTGATGAAGAAATCGAAAAACTGAG62150.15504185381661156No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC59000.14718373894095063No Hit
ATTCGATCCGTATACCCTGGCAATTGAGATCGGAAGAGCACACGTCTGAA58660.14633556146230786Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCAC58180.1451381344336357No Hit
ATTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAGATCGGAAGAGCACAC55980.13964992721888841No Hit
ACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAGATCGGAAGA55080.13740475154012816No Hit
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC54300.13545893261853592No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT53570.13363784567909703Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC52090.12994577900735793No Hit
AGGTTTTCGTCGTGCAGTTAGCGAACAGGATGAGATCGGAAGAGCACACG50920.1270270506249696No Hit
AACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAGATCGGAAG50640.12632855152491085No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAG50070.12490660692836268No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAGATCGGAAGAGCAC50040.12483176773907068No Hit
ATTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGA48740.12158873620308362No Hit
TGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACACGTCT48380.12069066593157951Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG47150.11762225917060715No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG46770.1166742961062417No Hit
AGGAACGTGATGGTAATGCAGTTCTGAATCTGCTGAGATCGGAAGAGCAC45910.11452890601320413No Hit
ACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGATCGGAAGAGCA45470.11343126457025467No Hit
AGGACTGGATGGATTCCAGTAACCCCGGAACCTGGACGGATGAGATCGGA45050.11238351592016654No Hit
ATGCGCGCGGCGATACCGAAGAACTTACCGAGATCGGAAGAGCACACGTC44400.11076200015217301No Hit
ATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGA44270.11043769699857432No Hit
ACGGTCTGGGTAAATTCACCGGGGCTAACACCCTGGAGATCGGAAGAGCA43100.10751896861618598No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA42240.10537357852314838No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT40350.10065870959775182No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)