FastQCFastQC Report
Fri 14 Jul 2023
SRR9691771_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691771_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4669594
Total Bases354.8 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAGATCGGAAGAGCACACG2027634.342197630029506No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA758701.624766521457754No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC705141.5100670422310805Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGAGATCGGAAGAGCA663271.4204018593479433No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC637351.364893821604191No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG533931.14341846421766No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA508431.0888098622706814Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG339930.7279647866602535No Hit
ATTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAGATCGGAAGAGCACAC333390.7139592863962049No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA219560.47019077033249573Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA196760.42136425565049124No Hit
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAGATCGGAAGAGCACAC178240.38170342004037183No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA167510.35872497694660394No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT158580.3396012586961522Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGAGATCGGAAGAGC156990.3361962517512229No Hit
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC152650.3269020818512273Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA151000.3233685840781875Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTTTCTGGCAAGCCTGGCATTTCGTGAGATCGGAAGAGCACACGTCTGA150300.3218695244168979Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT149900.32101291889616096No Hit
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG149620.3204132950316452Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGATCGGAAGAGCACA145380.3113332765118338No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA145320.31120478568372323No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT144090.30857072370745725No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG137380.2942011660970954No Hit
ATTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAGATCGGAAGAGCACA125750.2692953605816694No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA114010.24415398854804082Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCATCATTCTTCTGGTGTAGATCTGAGATCGGAAGAGCACACGTCTGAA110120.23582349985887424Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCCATCTCGTATG108060.23141198142707908TruSeq Adapter, Index 20 (97% over 39bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT103280.22117554545427293Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT101280.21689251785058827Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC99420.21290930217916162No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT99190.2124167540047379No Hit
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA98680.2113245819657983No Hit
ATCATCATTCTTCTGGTGTAGATCTGGAGATCGGAAGAGCACACGTCTGA91050.19498483165774155Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA89420.1914941641607386No Hit
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC88710.18997368936143055Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGAACGAGATCGGAAG85150.18234990022687197No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG83440.17868791162572165No Hit
ACCCTGAAAGGTCTGTATGCAACCCAGATCGGAAGAGCACACGTCTGAAC80830.17309856060291323Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC80520.1724346913243421No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT79970.17125685873332885TruSeq Adapter, Index 18 (100% over 35bp)
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA77510.1659887347807968No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG75640.16198410397135168No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT75220.16108466817457792Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA74690.1599496658596015No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA74000.15847202133633032No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA70570.1511266289960112No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT68660.14703633763449242No Hit
TCCGATGCTGGCAGATCGTACCTTTGCACAGATCGGAAGAGCACACGTCT65800.14091160816122342Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC65170.13956245446606277No Hit
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC64780.1387272640833443Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC63420.13581480531283877No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG58350.12495733033749828Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC57460.12305138305385864No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA56770.12157373853058746Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC56760.12155232339256902Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGGAAGAGCACAC56360.12069571787183211No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA56110.12016033942137154No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC55860.11962496097091095Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG55800.11949647014280042No Hit
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG55170.11814731644763977No Hit
ACCCTGAAAGGTCTGTATGCAACCCATGCAAGATCGGAAGAGCACACGTC52390.11219390807851817No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA52050.1114657933858918Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC51850.11103749062552333No Hit
TCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGA50800.10878890113358891Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC50500.10814644699303623No Hit
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC49750.10654031164165449No Hit
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC48700.10429172214972007Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCGTATCAGGACCAGACCAGATCGGAAGAGCACACGTCTGAACTCCAG48510.10388483452737005Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)