FastQCFastQC Report
Fri 14 Jul 2023
SRR9691772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9691772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4762395
Total Bases361.9 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAGATCGGAAGAGCACACG2024404.250802379894989No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAA760801.597515535775592No Hit
ATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAAC695401.460189673473116Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGAGATCGGAAGAGCA661871.3897839217452563No Hit
AGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTC638881.3415098915566641No Hit
TTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAG535661.1247702049074049No Hit
TGTGGGCACTCGAAGATACGGAGATCGGAAGAGCACACGTCTGAACTCCA511371.0737664557433813Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAGATCGGAAGAGCACAC349840.7345883741268836No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGAGATCGG348160.7310607372970953No Hit
ACAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGA225660.4738372184583597Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCA196070.41170461500988476No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA181010.3800818705714247No Hit
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAGATCGGAAGAGCACAC180440.3788849937898893No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGA176420.3704438628043243No Hit
ATTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGAGATCGGAAGAGC163520.343356651432735No Hit
AGCAATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCT162410.34102589138448197Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAAC156400.3284061905826795Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCT149790.31452661948452404No Hit
AATTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAA149710.3143586367783437Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTTTCTGGCAAGCCTGGCATTTCGTGAGATCGGAAGAGCACACGTCTGA148000.31076800643373764Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTG147840.31043204102137684Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGTCTTCTGACGTTCAGACTCTGAACGCAGATCGGAAGAGCACACGT147090.3088572031509356No Hit
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGATCGGAAGAGCACA143160.3006050527098235No Hit
TAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAG137000.2876703843339328No Hit
ATTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAGATCGGAAGAGCACA128140.26906629962445366No Hit
TGTGGGCACTCGAAGATACGGATTCTTAGATCGGAAGAGCACACGTCTGA113010.23729657031808576Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCCATCTCGTATG110730.2325090631919444TruSeq Adapter, Index 20 (97% over 39bp)
ATCATCATTCTTCTGGTGTAGATCTGAGATCGGAAGAGCACACGTCTGAA110590.2322150934561287Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACT105900.22236710730630282Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTCGTACTGAAACATCTTAATCAGATCGGAAGAGCACACGTCTGAACT104000.21837751803451835Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGATCGGA102030.214240943894826No Hit
AGCTGTCTTCTGACGTTCAGACTCTGAACGAGATCGGAAGAGCACACGTC101200.21249812331820436No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT100750.21155322059593965No Hit
TCCTCTGCAGTTCAGTCGGTAGAACGGCGGACTGAGATCGGAAGAGCACA96980.20363703556718835No Hit
ATCATCATTCTTCTGGTGTAGATCTGGAGATCGGAAGAGCACACGTCTGA92420.19406202131490563Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGAACTCC88720.18629282115406218Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCTGAAAGGTCTGTATGCAACCCAGATCGGAAGAGCACACGTCTGAAC85430.1793845323623933Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCGCAGCTGGAAGATGTTAGCCGTTTTCTGAAAGAACGAGATCGGAAG84760.17797767719813246No Hit
TCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCAGATCGGAAGAG84480.17738973772650107No Hit
CACTCGCGGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT83700.17575190634124216TruSeq Adapter, Index 18 (100% over 35bp)
AAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGC80840.16974652459529294No Hit
CTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGCCGAGATCGGAAGAG78140.16407710826170446No Hit
TCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGCACA76290.16019250818128272No Hit
AAACTGCGACGACACATTCACCTTGAACCAGATCGGAAGAGCACACGTCT76000.15958357087137878Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCA73880.15513202915759822No Hit
ACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGGA73360.15404014156742563No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGAAGATCGGAAGAGCACACGT72190.15158339448953728No Hit
ACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAA70900.1488746733523784No Hit
TCCGATGCTGGCAGATCGTACCTTTGCACAGATCGGAAGAGCACACGTCT66860.14039154669026824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTGCGGCGGCATCTCGGAGAGATCGGAAGAGCACACGTCTGAACTCC66510.13965662235072898Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAGC65900.13837575421610346No Hit
GTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCC63310.13293731410351303No Hit
TGTGGGCACTCGAAGATACGAGATCGGAAGAGCACACGTCTGAACTCCAG59430.12479015285376371Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCAC58520.12287934957096168No Hit
CAGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAA58490.12281635605614402Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGCGGCGGCATCTCGGAGAAGATCGGAAGAGCACACGTCTGAACTC58300.12241739712896557Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGTGAACGCAATGCGTTCCGACGTTCAGGCTGAGATCGGAAGAGCACAC58020.12182945765733418No Hit
AAGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACA57100.1198976565362596No Hit
AACACCTGATCGTCGAGCTTTACTCCAAGTAAAGCTTAGTACCAGATCGG56900.11947769977080859No Hit
TGTGGGCACTCGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC56690.11903674516708505Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTAGATCGGAAGAG56330.11828082298927325No Hit
ACCCTGAAAGGTCTGTATGCAACCCATGCAAGATCGGAAGAGCACACGTC54250.11391327262858289No Hit
CAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGC54010.11340932451004168No Hit
TTTCTCTGAGATGTTCGCAAGCGGGCCAGATCGGAAGAGCACACGTCTGA52130.10946173091480231Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGATCGGAAGAGCAC51980.10914676334071408No Hit
TCATCATTCTTCTGGTGTAGATCTGGGAGATCGGAAGAGCACACGTCTGA51100.10729895357272969Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGCGGCGGCATCTCGGAGATTAGATCGGAAGAGCACACGTCTGAAC49960.104905200009659Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCGTATCAGGACCAGACCAGATCGGAAGAGCACACGTCTGAACTCCAG49720.1044012518911178Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGAGATCAACAATGAAAGCAATTTTCGTACTGAGATCGGAAGAGCACAC48400.10162953723914124No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)